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Yongxiang Hong, Hong Wang, Mingju Xue, Peisheng Zhang,* Wanqiang Liu, Shu Chen, Rongjin Zeng, Jiaxi Cui, Yong Gao,* and Jian Chen*
Rational design of novel ratiometric and targetable fluorescent probes for selective imaging of lysosomal hypochlorous acid (HClO) is conducive to investigate the function of HClO in pathophysiological process. Herein, we for the first time combine 1- pyrenecarboxaldehyde (with π-π stacking effect) and 2-methylquinoline derivatives to synthesize an aggregation-induced-emission (AIE) probe (SA-C2-PCD) for HClO ratiometric detection in living cells. Subsequently, a kind of novel lysosome-targetable AIE dots (AIED-Lyso) with dual emission feature is prepared via a simple co-precipitation of lysosome-targeting surfactant MAACO720, amphiphilic block copolymers PEO113-b-PS46 and HClO-responsive probe (SA-C2-PCD). The free AIED-Lyso show only strong red AIE fluorescence without addition of HClO. However, when exposed to the strong oxidizing HClO, the SA-C2-PCD would release 1-pyrenecarboxaldehyde that aggregates through π-π stack interaction in aqueous solutions, inducing a distinct fluorescence change from red to blue. Interestingly, the as-prepared AIED-Lyso display good water dispersity, high selectivity and sensitivity (~ 70.4 nM), strong anti-interference ability, prominent long-term fluorescence stability (>10 weeks), and low cytotoxicity, and it can be successfully applied to visually detect the endogenous lysosomal HClO in live cells.
https://pubs.rsc.org/en/content/articlelanding/2019/qm/c8qm00511g#!divAbstract
Mater. Chem. Front., 2018, DOI: 10.1039/C8QM00511G
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