2017年12月23日New Phytologist在线文章，本文为中国农业大学张小兰课题组发表，文章揭示了CsLFY通过和WUSCHEL的相互作用调控黄瓜顶端分生组织维持的机理。

原题目为：

CsLFY is required for shoot meristem maintenance via interaction with WUSCHEL in cucumber (Cucumis sativus)

黄瓜是一种重要的蔬菜，具有不确定的生长习性，其中叶片由从顶端分生组织（SAM）产生，并且从叶腋生成单性花。有研究已表明，LEAFY（LFY）及其同系物在促进花发育和分枝方面发挥重要作用。

克隆了黄瓜中LFY同源基因CsLFY。分子生物学，发育生物学和生化工具相结合，研究了CsLFY在黄瓜中的生物学功能。

CsLFY在SAM，花分生组织和花器官原基中表达。 CsLFY的异位表达可以恢复拟南芥lfy-5突变体的表型。通过RNA干扰（RNAi）下调CsLFY，导致黄瓜芽发育缺陷和叶片起始过早中断。在CsLFY-RNAi株系中，CsWUS和可能的CsLFY靶基因（包括CsAP3和CUM1）的转录显著降低。进一步的生化分析表明，CsLFY与CsWUS在黄瓜中发生物理上的相互作用。

这些数据表明，CsLFY的新功能是，通过与CsWUS相互作用调节顶端分生组织维持，通过激活CsAP3和CUM1促进黄瓜花的发育。

Cucumber (Cucumis sativus) is an agronomically important vegetable with indeterminant growth habit, in which leaves are produced from the shoot apical meristem (SAM), and unisexual flowers are generated from the leaf axils. LEAFY (LFY) and its homologs have been shown to play important roles in promoting flower development and branching.

The LFY homolog gene CsLFY was cloned in cucumber. Molecular biology, developmental biology and biochemical tools were combined to explore the biological function of the LFY homologous gene CsLFY in cucumber.

CsLFY was expressed in the SAM, floral meristem and floral organ primordia. Ectopic expression of CsLFY rescued the phenotype of the lfy-5 mutant in Arabidopsis. Knockdown of CsLFY by RNA interference (RNAi) led to defective shoot development and premature discontinuance of leaf initiation in cucumber. Transcription of CsWUS and putative CsLFY target genes including CsAP3 and CUM1 were significantly reduced in the CsLFY-RNAi lines. Further biochemical analyses indicated that CsLFY physically interacts with CsWUS in cucumber.

These data suggested that CsLFY has a novel function in regulating shoot meristem maintenance through interaction with CsWUS, and promotes flower development via activation of CsAP3 and CUM1 in cucumber.

CsLFY调控黄瓜顶端分生组织及花发育的工作模式

CsLFY通过促进黄瓜SAM中CsWUS的活性，调控干细胞的维持和不定向生长。同时，CsLFY可能通过与CsWUS相互作用促进CUM1的表达，和/或促进CsAP3的转录，也可能与花分生组织中的CsUFO一起作用，进而促进花发育。

每日一词

unisexual

英['ju:nɪ'seksjʊəl]

美[ˌju:nɪ'sekʃʊrl]

adj. 单性的，雌雄异体的，雌雄异花的;