刚发表的一篇文章，把摘要和链接放在这里，有兴趣的同志，可以去看看。

http://link.springer.com/article/10.1007%2Fs11295-017-1106-3

Transcriptome analysis provides insights into wood formation during larch tree aging

Abstract

Tree age affects wood formation and yield. However, the underlying mechanisms are poorly understood, particularly at the molecular level.  树木年龄影响木材形成过程和产量， 本文旨在阐释 该 影响的分子机理。

In this study, we investigated the transcriptomic changes of the uppermost main stems of Larix kaempferi in an entire rotation period using the RNA-Seq method.  当年生顶端主干为材料，进行转录组测序和分析。

In total, ~151 million reads were obtained from the stems of 1-, 2-, 5-, 10-, 25-, and 50-year-old L. kaempferi trees. 六个年龄的落叶松，产生的数据大概是~151 million reads。

Combining these with the published Illumina sequencing reads, 299,637 assembled transcripts were generated, of which 161,232 were annotated. 和以前发表的数据一起组装，而后对组装好的序列进行注释。

12,927 transcripts were identified as differentially-expressed genes (DEGs) by time-series expression profiling; function enrichment analysis of these DEGs showed that 459 gene ontology terms in the biological process category were enriched. 根据年龄从小到大顺序，两两依次比较，得到差异表达的基因，随后进行功能富集分析，以鉴定这些基因都参与了哪些生物学过程。

These terms were associated with the processes of wood formation, such as cell differentiation, growth and death, and its hormonal regulation. 发现木材形成中的多个细胞学过程被富集，有 细胞分化，生长和死亡，以及激素对他们的调控。

Based on the expression patterns of L. kaempferi homologues of genes associated with ethylene, calcium, and cell-wall expansion and synthesis, the regulatory network of tracheid growth was outlined. 根据 乙烯，钙，细胞壁扩展 和 合成 相关基因的表达 模式， 勾勒出了 管状分子生长调控网络 模型。

Altogether, the comparative transcriptomic analysis reported here demonstrated the molecular aspects of aging effects on L. kaempferi wood formation. 就这样，落叶松 年龄 影响 木材形成的 分子机理 给大家 呈现啦。

The identification of genes involved in the regulatory network of tracheid growth provides a means of investigating the regulation of wood formation in gymnosperm trees and also offers potential targets for genetic manipulation to improve the properties of xylem fibers. 模型里的基因   有助于 研究  裸子植物 的 木材形成 过程，还有可能作为 候选基因 去 改变木材的品质。