平台 | 命令 | ||
Illumina 454 IonTorrent | 单 端 | 小于 ~70bp | bwa aln ref.fa reads.fq > reads.sai bwa samse ref.fa reads.sai reads.fq > aln-se.sam |
大于 ~70bp | bwa mem ref.fa reads.fq > aln.sam | ||
双 端 | 小于 ~70bp | bwa aln ref.fa read1.fq > read1.sai bwa aln ref.fa read2.fq > read2.sai bwa sampe ref.fa read1.sai read2.sai read1.fq read2.fq > aln-pe.sam | |
大于 ~70bp | bwa mem ref.fa read1.fq read2.fq > aln-pe.sam | ||
PacBio | bwa mem -x pacbio ref.fa reads.fq > aln.sam | ||
Oxford Nanopore | bwa mem -x ont2d ref.fa reads.fq > aln.sam |