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本文由清华大学 宋文飞整理
原文链接:
书籍:
冷冻电镜相关书籍:
《Three-Dimensional Electron Microscopy of Macromolecular Assemblies》
by Joachim Frank
冷冻电镜基本原理书籍:
《Diffraction Physics》Third Revised Edition by John M.Cowley
《电子衍射物理教程》 by 王蓉
《电子晶体学与图像处理》by 李方华
《薄晶体电子显微学》by P. Hirsch
视频:
冷冻电子显微镜(Cryo-EM)入门by 加州理工学院 Grant J.Jensen
链接:https://www.coursera.org/learn/cryo-em
Eva Nogales 的视频:
链接:https://www.youtube.com/watch?v=nkGRhYv01ag
MRC Lectures:
链接:ftp://ftp.mrc-lmb.cam.ac.uk/pub/scheres/EM-course
NRAMM Workshops:
链接:http://nramm.nysbc.org/workshops/
NRAMM videos:
链接:https://www.youtube.com/channel/UCgd1dW4HQ_yvpRjFc670htw
CCPEM symposium:
链接:https://sas.stfc.ac.uk/vportal/
Advances in Cryo-EM related books:
Cryo-EM Part A-C & Recent advances in cryo-EM (Methods in Enzymology)
链接:http://www.sciencedirect.com/science/bookseries/00766879/481
http://www.sciencedirect.com/science/bookseries/00766879/482
http://www.sciencedirect.com/science/bookseries/00766879/483
http://www.sciencedirect.com/science/bookseries/00766879/579
Reviews of Cryo-EM:
"A primer to single-particle cryo-electron microscopy."
https://www.ncbi.nlm.nih.gov/pubmed/25910204
"Structural analysis of macromolecular assemblies by electron microscopy." https://www.ncbi.nlm.nih.gov/pubmed/21919528
Structural Analysis of Protein Complexes by Cryo Electron Microscopy
https://link.springer.com/protocol/10.1007%2F978-1-4939-7033-9_28
电子显微三维重构技术发展与前沿
http://www.cnki.com.cn/Article/CJFDTotal-SWWL201007005.htm
论坛:
http://www.bio3dem.com/
数据库:
http://www.rcsb.org/pdb/home/home.do
http://www.ebi.ac.uk/pdbe/emdb/index.html/
http://www.emdatabank.org
软件:
常用计算相关软件:
Relion:
http://www2.mrc-lmb.cam.ac.uk/relion
Eman:
http://blake.bcm.edu/emanwiki/EMAN2
Gctf / Gautomatch:
http://www.mrc-lmb.cam.ac.uk/kzhang
Frealign:
http://emlab.rose2.brandeis.edu/frealign
数据收集、后处理等更多相关软件汇总网址:
http://www.emdatabank.org/emsoftware.html
https://en.wikibooks.org/wiki/Software_Tools_For_Molecular_Microscopy
致谢:
BioDEM 论坛Ming、Jyfhat123为本文提供了资料,Bio3DEM 张凯(MRC-LMB)对本文撰写和校对等提出宝贵意见,特此致谢!
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冷冻电镜(cryo-EM)相关链接:
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