||
illumina
illumina的小RNA测序adapter
illumina的小RNA测序,有多种adapter。如下表所示:
1. Illumina Single End Adapter 1 ACACTCTTTCCCTACACGACGCTGTTCCATCT
2. Illumina Single End Adapter 2 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT
3. Illumina Single End PCR Primer 1
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
4. Illumina Single End PCR Primer 2 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT
5. Illumina Single End Sequencing Primer ACACTCTTTCCCTACACGACGCTCTTCCGATCT
6.
7. Illumina Paired End Adapter 1 ACACTCTTTCCCTACACGACGCTCTTCCGATCT
8. Illumina Paired End Adapter 2 CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
9. Illumina Paried End PCR Primer 1
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
10. Illumina Paired End PCR Primer 2
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
11. Illumina Paried End Sequencing Primer 1 ACACTCTTTCCCTACACGACGCTCTTCCGATCT
12. Illumina Paired End Sequencing Primer 2 CGGTCTCGGCATTCCTACTGAACCGCTCTTCCGATCT
13.
14. Illumina DpnII expression Adapter 1 ACAGGTTCAGAGTTCTACAGTCCGAC
15. Illumina DpnII expression Adapter 2 CAAGCAGAAGACGGCATACGA
16. Illumina DpnII expression PCR Primer 1 CAAGCAGAAGACGGCATACGA
17. Illumina DpnII expression PCR Primer 2
AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
18. Illumina DpnII expression Sequencing Primer CGACAGGTTCAGAGTTCTACAGTCCGACGATC
19.
20. Illumina NlaIII expression Adapter 1 ACAGGTTCAGAGTTCTACAGTCCGACATG
21. Illumina NlaIII expression Adapter 2 CAAGCAGAAGACGGCATACGA
22. Illumina NlaIII expression PCR Primer 1 CAAGCAGAAGACGGCATACGA
23. Illumina NlaIII expression PCR Primer 2
AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
24. Illumina NlaIII expression Sequencing Primer CCGACAGGTTCAGAGTTCTACAGTCCGACATG
25.
26. <font class="Apple-style-span" color="#0000ff">Illumina Small RNA Adapter 1
GTTCAGAGTTCTACAGTCCGACGATC
27. Illumina Small RNA Adapter 2 TCGTATGCCGTCTTCTGCTTGT
28. Illumina Small RNA RT Primer CAAGCAGAAGACGGCATACGA
29. Illumina Small RNA PCR Primer 1 CAAGCAGAAGACGGCATACGA
30. Illumina Small RNA PCR Primer 2
AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
31. Illumina Small RNA Sequencing Primer
CGACAGGTTCAGAGTTCTACAGTCCGACGATC</font>
32.
33. Illumina Multiplexing Adapter 1 GATCGGAAGAGCACACGTCT
34. Illumina Multiplexing Adapter 2 ACACTCTTTCCCTACACGACGCTCTTCCGATCT
35. Illumina Multiplexing PCR Primer 1.01
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
36. Illumina Multiplexing PCR Primer 2.01 GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
37. Illumina Multiplexing Read1 Sequencing Primer ACACTCTTTCCCTACACGACGCTCTTCCGATCT
38. Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC
39. Illumina Multiplexing Read2 Sequencing Primer GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
40.
41. Illumina PCR Primer Index 1
CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTC
42. Illumina PCR Primer Index 2
CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTC
43. Illumina PCR Primer Index 3
CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTC
44. Illumina PCR Primer Index 4
CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTC
45. Illumina PCR Primer Index 5
CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTC
46. Illumina PCR Primer Index 6
CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTC
47. Illumina PCR Primer Index 7
CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTC
48. Illumina PCR Primer Index 8
CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTC
49. Illumina PCR Primer Index 9
CAAGCAGAAGACGGCATACGAGATCTGATCGTGACTGGAGTTC
50. Illumina PCR Primer Index 10
CAAGCAGAAGACGGCATACGAGATAAGCTAGTGACTGGAGTTC
51. Illumina PCR Primer Index 11
CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTC
52. Illumina PCR Primer Index 12
CAAGCAGAAGACGGCATACGAGATTACAAGGTGACTGGAGTTC
53.
54. Illumina DpnII Gex Adapter 1 GATCGTCGGACTGTAGAACTCTGAAC
55. Illumina DpnII Gex Adapter 1.01 ACAGGTTCAGAGTTCTACAGTCCGAC
56. Illumina DpnII Gex Adapter 2 CAAGCAGAAGACGGCATACGA
57. Illumina DpnII Gex Adapter 2.01 TCGTATGCCGTCTTCTGCTTG
58. Illumina DpnII Gex PCR Primer 1 CAAGCAGAAGACGGCATACGA
59. Illumina DpnII Gex PCR Primer 2
AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
60. Illumina DpnII Gex Sequencing Primer CGACAGGTTCAGAGTTCTACAGTCCGACGATC
61.
62. Illumina NlaIII Gex Adapter 1.01 TCGGACTGTAGAACTCTGAAC
63. Illumina NlaIII Gex Adapter 1.02 ACAGGTTCAGAGTTCTACAGTCCGACATG
64. Illumina NlaIII Gex Adapter 2.01 CAAGCAGAAGACGGCATACGA
65. Illumina NlaIII Gex Adapter 2.02 TCGTATGCCGTCTTCTGCTTG
66. Illumina NlaIII Gex PCR Primer 1 CAAGCAGAAGACGGCATACGA
67. Illumina NlaIII Gex PCR Primer 2
AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
68. Illumina NlaIII Gex Sequencing Primer CCGACAGGTTCAGAGTTCTACAGTCCGACATG
69.
70. Illumina Small RNA RT Primer CAAGCAGAAGACGGCATACGA
71. Illumina 5p RNA Adapter GTTCAGAGTTCTACAGTCCGACGATC
72. Illumina RNA Adapter1 TCGTATGCCGTCTTCTGCTTGT
73.
74. Illumina Small RNA 3p Adapter 1 ATCTCGTATGCCGTCTTCTGCTTG
75. Illumina Small RNA PCR Primer 1 CAAGCAGAAGACGGCATACGA
76. Illumina Small RNA PCR Primer 2
AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
77. Illumina Small RNA Sequencing Primer CGACAGGTTCAGAGTTCTACAGTCCGACGATC
78.
79. TruSeq Universal Adapter
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
80. TruSeq Adapter, Index 1
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG
81. TruSeq Adapter, Index 2
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG
82. TruSeq Adapter, Index 3
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTG
83. TruSeq Adapter, Index 4
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG
84. TruSeq Adapter, Index 5
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG
85. TruSeq Adapter, Index 6
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTG
86. TruSeq Adapter, Index 7
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTG
87. TruSeq Adapter, Index 8
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTG
88. TruSeq Adapter, Index 9
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTG
89. TruSeq Adapter, Index 10
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTG
90. TruSeq Adapter, Index 11
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTG
91. TruSeq Adapter, Index 12
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTG
92.
93. Illumina RNA RT Primer GCCTTGGCACCCGAGAATTCCA
94. Illumina RNA PCR Primer
AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGA
95.
96. RNA PCR Primer, Index 1
CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
97. RNA PCR Primer, Index 2
CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
98. RNA PCR Primer, Index 3
CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
99. RNA PCR Primer, Index 4
CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
100. RNA PCR Primer, Index 5
CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
101. RNA PCR Primer, Index 6
CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
102. RNA PCR Primer, Index 7
CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
103. RNA PCR Primer, Index 8
CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
104. RNA PCR Primer, Index 9
CAAGCAGAAGACGGCATACGAGATCTGATCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
105. RNA PCR Primer, Index 10
CAAGCAGAAGACGGCATACGAGATAAGCTAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
106. RNA PCR Primer, Index 11
CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
107. RNA PCR Primer, Index 12
CAAGCAGAAGACGGCATACGAGATTACAAGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
108. RNA PCR Primer, Index 13
CAAGCAGAAGACGGCATACGAGATTTGACTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
109. RNA PCR Primer, Index 14
CAAGCAGAAGACGGCATACGAGATGGAACTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
110. RNA PCR Primer, Index 15
CAAGCAGAAGACGGCATACGAGATTGACATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
111. RNA PCR Primer, Index 16
CAAGCAGAAGACGGCATACGAGATGGACGGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
112. RNA PCR Primer, Index 17
CAAGCAGAAGACGGCATACGAGATCTCTACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
113. RNA PCR Primer, Index 18
CAAGCAGAAGACGGCATACGAGATGCGGACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
114. RNA PCR Primer, Index 19
CAAGCAGAAGACGGCATACGAGATTTTCACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
115. RNA PCR Primer, Index 20
CAAGCAGAAGACGGCATACGAGATGGCCACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
116. RNA PCR Primer, Index 21
CAAGCAGAAGACGGCATACGAGATCGAAACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
117. RNA PCR Primer, Index 22
CAAGCAGAAGACGGCATACGAGATCGTACGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
118. RNA PCR Primer, Index 23
CAAGCAGAAGACGGCATACGAGATCCACTCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
119. RNA PCR Primer, Index 24
CAAGCAGAAGACGGCATACGAGATGCTACCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
120. RNA PCR Primer, Index 25
CAAGCAGAAGACGGCATACGAGATATCAGTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
121. RNA PCR Primer, Index 26
CAAGCAGAAGACGGCATACGAGATGCTCATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
122. RNA PCR Primer, Index 27
CAAGCAGAAGACGGCATACGAGATAGGAATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
123. RNA PCR Primer, Index 28
CAAGCAGAAGACGGCATACGAGATCTTTTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
124. RNA PCR Primer, Index 29
CAAGCAGAAGACGGCATACGAGATTAGTTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
125. RNA PCR Primer, Index 30
CAAGCAGAAGACGGCATACGAGATCCGGTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
126. RNA PCR Primer, Index 31
CAAGCAGAAGACGGCATACGAGATATCGTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
127. RNA PCR Primer, Index 32
CAAGCAGAAGACGGCATACGAGATTGAGTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
128. RNA PCR Primer, Index 33
CAAGCAGAAGACGGCATACGAGATCGCCTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
129. RNA PCR Primer, Index 34
CAAGCAGAAGACGGCATACGAGATGCCATGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
130. RNA PCR Primer, Index 35
CAAGCAGAAGACGGCATACGAGATAAAATGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
131. RNA PCR Primer, Index 36
CAAGCAGAAGACGGCATACGAGATTGTTGGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
132. RNA PCR Primer, Index 37
CAAGCAGAAGACGGCATACGAGATATTCCGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
133. RNA PCR Primer, Index 38
CAAGCAGAAGACGGCATACGAGATAGCTAGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
134. RNA PCR Primer, Index 39
CAAGCAGAAGACGGCATACGAGATGTATAGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
135. RNA PCR Primer, Index 40
CAAGCAGAAGACGGCATACGAGATTCTGAGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
136. RNA PCR Primer, Index 41
CAAGCAGAAGACGGCATACGAGATGTCGTCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
137. RNA PCR Primer, Index 42
CAAGCAGAAGACGGCATACGAGATCGATTAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
138. RNA PCR Primer, Index 43
CAAGCAGAAGACGGCATACGAGATGCTGTAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
139. RNA PCR Primer, Index 44
CAAGCAGAAGACGGCATACGAGATATTATAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
140. RNA PCR Primer, Index 45
CAAGCAGAAGACGGCATACGAGATGAATGAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
141. RNA PCR Primer, Index 46
CAAGCAGAAGACGGCATACGAGATTCGGGAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
142. RNA PCR Primer, Index 47
CAAGCAGAAGACGGCATACGAGATCTTCGAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
143. RNA PCR Primer, Index 48
CAAGCAGAAGACGGCATACGAGATTGCCGAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
144.
145. ABI Dynabead EcoP Oligo CTGATCTAGAGGTACCGGATCCCAGCAGT
146. ABI Solid3 Adapter A
CTGCCCCGGGTTCCTCATTCTCTCAGCAGCATG
147. ABI Solid3 Adapter B
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT
148. ABI Solid3 5' AMP Primer CCACTACGCCTCCGCTTTCCTCTCTATG
149. ABI Solid3 3' AMP Primer CTGCCCCGGGTTCCTCATTCT
150. ABI Solid3 EF1 alpha Sense Primer CATGTGTGTTGAGAGCTTC
151. ABI Solid3 EF1 alpha Antisense Primer GAAAACCAAAGTGGTCCAC
152. ABI Solid3 GAPDH Forward Primer TTAGCACCCCTGGCCAAGG
153. ABI Solid3 GAPDH Reverse Primer CTTACTCCTTGGAGGCCATG
Illumina Small RNA Adapter 1 GTTCAGAGTTCTACAGTCCGACGATC
Illumina Small RNA Adapter 2 TCGTATGCCGTCTTCTGCTTGT
Illumina Small RNA RT Primer CAAGCAGAAGACGGCATACGA
Illumina Small RNA PCR Primer 1 CAAGCAGAAGACGGCATACGA
Illumina Small RNA PCR Primer 2
AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
Illumina Small RNA Sequencing Primer
CGACAGGTTCAGAGTTCTACAGTCCGACGATC
引用:http://www.seq.cn/forum.php?mod=viewthread&tid=3027&extra=
Archiver|手机版|科学网 ( 京ICP备07017567号-12 )
GMT+8, 2024-10-11 09:49
Powered by ScienceNet.cn
Copyright © 2007- 中国科学报社