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2016年5月26日,黑树莓(black raspberry,Rubus occidentalis)基因组论文在《Plant Journal》在线发表。
论文链接:http://onlinelibrary.wiley.com/doi/10.1111/tpj.13215/full
DOI:10.1111/tpj.13215
黑树莓属于蔷薇科,而在蔷薇科之中已经测序了6个物种,也就是说这是蔷薇科第七个测序的物种。之前已经完成全基因组测序的蔷薇科物种包括:草莓、苹果、桃、梅、中国梨、欧洲梨。
黑树莓基因组草图243Mb,28005个预测基因,与草莓基因组有较好的同线性关系。
论文摘要:
Black raspberry (Rubus occidentalis) is an important specialty fruit crop in the U.S. Pacific Northwest that can hybridize with the globally commercialized red raspberry (R. idaeus). Here we report a 243 Mb draft genome of black raspberry that will serve as a useful reference for the Rosaceae and Rubus fruit crops (raspberry, blackberry, and their hybrids). The black raspberry genome is largely collinear to the diploid woodland strawberry (Fragaria vesca) with a conserved karyotype and few notable structural rearrangements. Centromeric satellite repeats are widely dispersed across the black raspberry genome, in contrast to the tight association with the centromere observed in most plants. Among the 28,005 predicted protein-coding genes, we identified 290 very recent small-scale gene duplicates enriched for sugar metabolism, fruit development, and anthocyanin related genes which may be related to key agronomic traits during black raspberry domestication. This contrasts patterns of recent duplications in the wild woodland strawberry F. vesca, which show no patterns of enrichment, suggesting gene duplications contributed to domestication traits. Expression profiles from a fruit ripening series and roots exposed to Verticillium dahliae shed insight into fruit development and disease response, respectively. The resources presented here will expedite the development of improved black and red raspberry, blackberry and other Rubus cultivars.
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