siRNA targeting stathmin inhibits invasion and enhances chemotherapy sensitivity of stem cells derived from glioma cell lines

Yuwen Song, Luyan Mu, Xuezhe Han, Xiaoqian Liu and Songbin Fu
Acta Biochim Biophys Sin (Shanghai). 2014 Dec;46(12):1034-40. doi: 10.1093/abbs/gmu099Department of Neurosurgery, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China  

Glioma is one of the most highly angiogenic tumors, and glioma stem cells (GSCs) are responsible for resistance to chemotherapy and radiotherapy, as well as recurrence after operation. Stathmin is substantial for mitosis and plays an important role in proliferation and migration of glioma-derived endothelial cells. However, the relationship between stathmin and GSCs is incompletely understood. Here we isolated GSCs from glioma cell lines U87MG and U251, and then used siRNA targeting stathmin for silencing. We showed that silencing of stathmin suppressed the proliferation, increased the apoptosis rate, and arrested the cell cycle at G2/M phase in GSCs. Silencing of stathmin in GSCs also resulted in inhibited the migration/invasion as well as the capability of vasculogenic mimicry. The susceptibility of GSCs to temozolomide was also enhanced by stathmin silencing. Our findings suggest stathmin as a potential target in GSCs for glioma treatment.

图例: stathmin反义RNA抑制细胞增殖、诱导细胞凋亡

全文: http://abbs.oxfordjournals.org/content/46/12/1034.full