Curculigoside isolated from Curculigo orchioides prevents hydrogen peroxide-induced dysfunction and oxidative damage in calvarial osteoblasts

Ying Wang, Lu Zhao, Yin Wang, Jinlong Xu, Yan Nie, Yuanhui Guo, Yongtao Tong, Luping Qin, and Qiaoyan Zhang

Acta Biochim Biophys Sin (Shanghai). 2012 May;44(5):431-41. doi: 10.1093/abbs/gms014

School of Pharmacy, Second Military Medical University, Shanghai, China

Reactive oxygen species (ROS), including H₂O₂, play a critical role in the pathophysiology of osteoporosis. Therefore, agents or antioxidants that can inhibit ROS production have a high clinical value in the treatment of osteoporosis. Curculigoside (CUR), one of the main bioactive phenolic compounds isolated from the rhizome of Curculigoorchioides Gaertn., is reported to have potent antioxidant and anti-osteoporotic properties. However, there is no direct evidence to link the antioxidant capacity of CUR with the observed anti-osteoporotic effect, and relevant molecular mechanisms remain unclear. Therefore, we investigated the protective effects of CUR against oxidative stress in calvarialosteoblasts and discussed the related mechanisms. It was found that osteoblast viability decreased significantly after 48-h exposure to 400 μM of H₂O₂, compared with vehicle-treated cells, and the cytotoxic effect of H₂O₂ was reversed significantly when pretreated with 0.1-10 μM of CUR (P< 0.05). Pretreatment with 0.1-10 μM of CUR decreased ROS production and lipid peroxidation, and increased the activities of antioxidant enzymes, such as superoxide dismutase and glutathione peroxidase in osteoblasts induced by H₂O₂. In addition, H₂O₂-induced reduction of differentiation markers such as alkaline phosphatase, calcium deposition, and Runx2 level was significantly recovered in the presence of CUR. CUR also reversed H₂O₂-induced stimulation of extracellular signal-regulated kinase 1/2, and nuclear factor-κB signaling and the inhibition of p38 mitogen-activated protein kinase activation. These results provide new insights into the osteoblast-protective mechanisms of CUR through reducing the production of ROS, suggesting that CUR may be developed as a bio-safe agent for the prevention and treatment of osteoporosis and other bone-related human diseases.

图例: 仙茅甙抑制H₂O₂诱导的细胞损伤

全文: http://abbs.oxfordjournals.org/content/44/5/431.full.pdf+html