Enhanced tenogenic differentiation and tendonlike tissue formation by CHIP overexpression in tendon-derived stem cells

Weifeng Han, Lei Chen, Junpeng Liu, and Ai Guo

Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China

Acta Biochim Biophys Sin 2017, 49: 311–317; doi: 10.1093/abbs/gmx005

The carboxyl terminus of Hsc70-interacting protein (CHIP, also known as STUB1) plays critical roles in the proliferation and differentiation of many types of cells. The potential function of CHIP in tendon-derived stem cells (TDSCs) remains largely unknown at present. Here, we investigated the effects of CHIP on tenogenic differentiation of TDSCs via lentivirus-mediated overexpression. Forced expression of CHIP induced morphological changes and significantly enhanced cell proliferation, as well as tendon differentiation in vitro. Upon stimulation with differentiation induction medium, CHIP-overexpressing TDSCs displayed significant inhibition of differentiation into osteogenic and adipogenic lineages. Subsequent implantation of TDSCs overexpressing CHIP with collagen sponges into nude mice induced a marked increase in ectopic tendon formation in vivo, compared with the control group. Our findings collectively suggest that CHIP is an important contributory factor to tenogenic tissue formation.

CHIP overexpression enhances TDSC proliferation and tenogenic differentiation

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