Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5′-phosphate oxidase

Jing Gu , Yuanyuan Chen, Hongsen Guo, Manluan Sun, Mingkun Yang , Xude Wang , Xian'en Zhang, and Jiaoyu Deng

Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

Acta Biochim Biophys Sin 2017, 49: 186–192; doi: 10.1093/abbs/gmw129

Nɛ-lysine acetylation is one of the most abundant post-translational modifications in eukaryote and prokaryote. Protein acetylome of Escherichia coli has been screened using mass spectrometry (MS) technology, and many acetylated proteins have been identified, including the pyridoxine 5′-phosphate oxidase (EcPNPOx), but the biological roles played by lysine acetylation in EcPNPOx still remain unknown. In this study, EcPNPOx was firstly overexpressed and purified, and two acetylated lysine residues were identified by the subsequent liquid chromatography–tandem mass spectrometry analysis. Site-directed mutagenesis analysis demonstrated that acetylated lysine residues play important roles in the enzymatic activity and enzymatic properties of the protein. EcPNPOx could be non-enzymatically acetylated by acetyl-phosphate and deacetylated by CobB in vitro. Furthermore, enzymatic activities of acetylated and deacetylated EcPNPOx were compared in vitro, and results showed that acetylation led to a decrease of its enzymatic activity, which could be rescued by CobB deacetylation. Taken together, our data suggest that CobB modulates the enzymatic activity of EcPNPOx in vitro.

Stereoview of the active site of EcPNPOx

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