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已有 4846 次阅读 2017-8-4 09:45 |系统分类:科研笔记

DNA-guided genome editing using the Natronobacterium gregoryi Argonaute

作者:Gao, F (Gao, Feng)^{[ 1 ]}; Shen, XZ (Shen, Xiao Z.)^{[ 2 ]}; Jiang, F (Jiang, Feng)^{[ 1 ]}; Wu, YQ (Wu, Yongqiang)^{[ 1 ]}; Han, CY (Han, Chunyu)^{[ 1 ]}

NATURE BIOTECHNOLOGY

卷: 34

期: 7

页: 768-+

DOI: 10.1038/nbt.3547

出版年: JUL 2016

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摘要

The RNA-guided endonuclease Cas9 has made genome editing a widely accessible technique. Similar to Cas9, endonucleases from the Argonaute protein family also use oligonucleotides as guides to degrade invasive genomes. Here we report that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5' phosphorylated single-stranded guide DNA (gDNA) of similar to 24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA. The NgAgo-gDNA system does not require a protospacer-adjacent motif (PAM), as does Cas9, and preliminary characterization suggests a low tolerance to guide-target mismatches and high efficiency in editing (G+C)-rich genomic targets.

关键词

KeyWords Plus:CRISPR-CAS9 SYSTEM; FOREIGN DNA; HUMAN-CELLS; CAS9; ENDONUCLEASE; SPECIFICITY; NUCLEASE; PROTEINS; CLEAVAGE; REVEALS

作者信息

通讯作者地址: Han, CY (通讯作者)

Hebei Univ Sci & Technol, Dept Biosci & Bioengn, Shijiazhuang, Hebei, Peoples R China.

地址:

	[ 1 ] Hebei Univ Sci & Technol, Dept Biosci & Bioengn, Shijiazhuang, Hebei, Peoples R China
	[ 2 ] Zhejiang Univ, Dept Physiol, Sch Med, Hangzhou, Zhejiang, Peoples R China

电子邮件地址:hanchunyu74@aliyun.com

基金资助致谢