Herbaceous plants contribute   substantially to the diversity and abundance of forests ecosystems,   particularly in temperate regions (Gilliam, 2007, 2014; Luo et al., 2016a,   2016b; Wang, Tang & Fang, 2007). However, interactions between herbaceous   and woody plant layers in community assemblages have long been neglected in   forest dynamics models, even though it is widely known that herbaceous   species can impact the regeneration of tree species (Murphy, Salpeter &   Comita, 2016; Thrippleton, Bugmann, Kramer-Priewasser & Snell, 2016). DNA   barcoding has mainly been used for tree species in forest dynamic plots of   tropical, subtropical, and temperate regions (Erickson et al., 2014; Kress et   al., 2009; Pei et al., 2015). Thus in many cases, the herbaceous component of   the community remains underexplored. In this study, we use DNA barcodes to   identify and document the herbaceous and woody flora of a 25-ha subalpine   spruce-fir forest dynamics plot on the Yulong Mountain, northwestern Yunnan,   China. We aim to address the following three questions: (a) What barcode   strategy provides the highest species resolution in a subalpine forest? (b)   Do species discrimination rates vary among herbaceous and woody species in   this community? (c) Does the number of species within a genus affect species   resolution rates in high elevation forest dynamic plots.

草本植物对森林生态系统的多样性和丰富性做出了重大贡献，特别是在温带地区（Gilliam, 2007, 2014; Luo et al., 2016a, 2016b; Wang, Tang &   Fang, 2007）。尽管众所周知草本物种会影响树种的再生（Murphy, Salpeter & Comita, 2016; Thrippleton, Bugmann,   Kramer-Priewasser & Snell, 2016），但在森林动态模型中，群落组成中草本和木本植物层之间的相互作用却一直被忽视。DNA条形码主要用于研究热带、亚热带和温带森林动态样地的树种（Erickson et al., 2014; Kress et al., 2009; Pei et al., 2015）。因此，在许多情况下，该群落的草本成分仍有待研究。本文利用DNA条形码对滇西北玉龙雪山25公顷亚高山云杉-冷杉森林动态样地的草本和木本植物区系进行了鉴定和记录。我们的目标是解决以下三个问题：（a）什么条形码策略提供了亚高山森林中最高的物种识别分辨率？（b）该群落中草本和木本物种的物种识别率是否不同？（c）一个属内的物种数量是否影响高海拔森林动态图中的物种分辨率？