题目：SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis

主要是发现了早期结肠癌的一个生物标记分子，可以帮助我们对于早期的结肠癌进行诊断，得到有效的治疗，以免以后恶化。

路线图：SPG20甲基化-------spartin蛋白表达下降--------胞质分裂停滞

前面的英文句型：

1 here we show that

2the present study show that

3In a previous epigenome study, we identified a list of genes

4.。。gene located in the chromosome band

5 。。。encode 。。。 which contain 。。domain and

6.。。is a multifunction proteins which found

7it hase also been reported that

8 in the present study， the promotor methylation ststuse of the

9 we show that the。。

结果分析

1Identification of SPG20 as a biomarker for colorectal cancer

by using。。。。。we found that

用了芯片筛选到方法，在正常和腺瘤及癌症的细胞中找出甲基化差异额基因启动子，发现了SPG20有差异，还用了数量特异的甲基化PCR检测。

2 Real-time quantitative analysis shows that SPG20 promoter hypermethylation causes loss of gene expression

用了实时反转录PCR来检测不同细胞的mRNA表达量，是第一个实验的延续，还用了表观遗传有关的药物处理

3Hypermethylated colon cancer cell lines do not express the SPG20 protein product Spartin

用了western blotting和免疫荧光检测技术来分析蛋白的表达，结果和对照相比没有蛋白的表达，也与前面的实验相对应。

3 Spartin localizes to the spindle poles and the cytokinesis bridge of dividing daughter cells

in summary ，detection and quantification

this should 被

 expored further

we further show that pomoter

with the loss off

our data not only identify a promising biomaker for

but also reveal a cellular mechanism that could be involved in the early

a number of additional roles  have been described for ，including int

in the present study ，we observed that even through colon cancer cell lines with convoluted