Effects of carrier, sterilisation method, and incubation on survival
of Bradyrhizobium japonicum in soybean (Glycine max L.) inoculants
Enzyme and Microbial Technology 41 (2007) 780–784
The production and quality of rhizobial inoculants in many developing
countries is limited by the availability of suitable carriers or
technologicallimitations. Experiments were conducted to evaluate the
potential of various inexpensive and widely available carrier materials.
The carriersevaluated, were: perlite with pH adjusted with calcium carbonate
or charcoal, 1:4 mixtures of perlite and malt residue, sugarcane bagasse,
coal, and rice husk.We also contrasted sterilisation procedures (autoclaving
or gamma irradiation) and incubation after injection (with or without
initialtwo weeks incubation at 28 ?C) for these various carriers. Survival
of Bradyrhizobium japonicum strain CB1809 was monitored over a period of
6 months upon storage at 4 ?C. Most carriers evaluated, were able to
maintain rhizobial populations of more than 1×109 rhizobia per gram of
inoculant over that time period, with mixtures of perlite with either
sugarcane bagasse or malt residue supporting the largest rhizobial
populations and a mixture of perlite and rice husk the lowest. All carriers
supported rhizobial growth over the 6 months period. Initially, rhizobial
populations were greater with gamma irradiation than autoclaving, however
after 6 months, this response was significant only with the perlite and
sugarcane bagasse mixture. The incubation of the inoculant after injection
also ultimately did not benefit rhizobial levels for any of the carriers,
tested. Using simple sterilisation procedures and without incubating after
injection, perlite based carriers can produce high quality inexpensive
inoculants, maintaining bacterial populations of more than 1×109/g rhizobia
for at least 6 months.
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