CNS翻译练习:Science. 2010 Aug 13;
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2010-8-25 23:28
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Science. 2010 Aug 13;329(5993):845-8.
An allosteric self-splicing ribozyme triggered by a bacterial second messenger.
Lee ER, Baker JL, Weinberg Z, Sudarsan N, Breaker RR.Department of Molecular, Cellular, and Developmental Biology, Yale University, Box 208103, New Haven, CT 06520-8103, USA.
Abstract
Group I self-splicing ribozymes commonly function as components of selfish mobile genetic elements. We identified an allosteric group I ribozyme, wherein self-splicing is regulated by a distinct riboswitch class that senses the bacterial second messenger c-di-GMP. The tandem RNA sensory system resides in the 5' untranslated region of the messenger RNA for a putative virulence gene in the pathogenic bacterium Clostridium difficile. c-di-GMP binding by the riboswitch induces folding changes at atypical splice site junctions to modulate alternative RNA processing. Our findings indicate that some self-splicing ribozymes are not selfish elements but are harnessed by cells as metabolite sensors and genetic regulators.
一个细菌第二信使引发一种可变构的自我剪切核酶I类自我剪切核酶一般作为自私移动遗传原件的组分。我们确定了一个I类自我剪切核酶的异构体,它的自我剪切是由一种特殊的核开关种类调控,这类核开关感受细菌的第二信使 c-di-GMP。该串联RNA感受系统 定位在病原菌艰难梭菌的一个推测的毒性基因的信使RNA5'非翻译区。 c-di-GMP被核开关结合后在一个非典型的拼接位点连接,调节了替代的RNA形成过程,引导了构象变化。我们的发现提示了一些自我剪切的核酶不是自私(移动遗传原件)的组分,而被细胞用于代谢物感应和遗传调控元件。