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云师大化工学院赵焱教授、杨建梅在top期刊《Talanta》发表最新研究成果

已有 550 次阅读 2024-9-22 22:28 |个人分类:云师大研究|系统分类:论文交流

2024年9月19日，Elsevier 旗下top期刊《Talanta》在线发表了云南师范大学化学与化工学院赵焱教授、杨建梅博士课题组最新研究成果《Target-promoted activation of DNAzyme walker for in situ assembly of hemin/G-quadruplex nanowires enable ultrasensitive and label-free electrochemical myocardial microRNA assay》。云南师范大学化学与化工学院赵焱教授、杨建梅博士为通讯作者。

https://www.sciencedirect.com/science/article/abs/pii/S003991402401302X

Abstract

The concentration elevation of myocardial microRNA (miRNA) biomarker is associated with the pathogenic process of acute myocardial infarction (AMI), and sensitive quantification of myocardial miRNA biomarker plays an important role for early AMI diagnosis and its treatment. In response, this work describes an ultrasensitive and non-label electrochemical biosensor for the assay of myocardial miRNA based on cascade signal amplifications integrated by DNAzyme walker and hemin/G-quadruplex nanowires. The DNAzyme walker is activated by presence of target miRNAs to move along the electrode surface to cyclically cleave the substrate hairpins to release G-quadruplex segments, which further trigger the in situ formation of many hemin/G-quadruplex nanowires. The large amounts of hemin intercalated into the DNA nanowires subsequently generate drastically magnified electrochemical current signals for highly sensitive label-free assay of myocardial miRNAs down to 15.7 fM within dynamic range of 100 fM to 10 nM. Such a biosensor also has high selectivity and can monitor myocardial miRNAs in diluted serums at low levels, providing a sensitive and reliable platform for diagnosing infarct-associated cardiovascular diseases.

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