2023年8月7日，Elsevier 旗下top期刊《Talanta》在线发表了云南师范大学化学与化工学院胡蓉教授课题组最新研究成果《Target-triggered stochastic DNAzyme motors on spherical nucleic acids for simultaneous fluorescence assay of double miRNAs》。云南师范大学化学化工学院胡蓉教授和杨通博士为共同通讯作者。

https://doi.org/10.1016/j.talanta.2023.125032

Abstract

Simultaneous quantifications of multiple miRNAs in the single-sampling system would be conducive to the accurate diagnosis of diseases in contrast with single miRNA analysis. In this work, a stochastic DNAzyme motor on spherical nucleic acids (SNAs) for simultaneous fluorescence assay of double miRNAs was established. Hairpin 1 (H1)-FAM-7a and H1-TAMRA-133a-functionalized magnetic beads (MBs) as SNAs were mixed. Targets (let-7a and miRNA-133a) reacted with two different S1 and S2, triggering the formation of two types of metal DNAzymes. The DNAzymes can further react with H1 stem-loop DNA on SNAs to release the two fluorescent DNA-FAM and DNA-TAMRA fragments in the presence of Mg2+. Meanwhile, the DNAzyme as DNA motors were separated from the previous H1 probe to participate the next cycling operations, resulting in the signal amplification toward the simultaneous and sensitive detection of let-7a and miRNA-133a. SNAs with three dimensional nanostructures provided enough space for the operation of DNAzyme walker, promoting the sensitivity of this proposed analytical system. The two mixed SNAs enable one-step and specific quantification of miRNA let-7a and miRNA-133a with lower detection limits of 90.5 fM and 74.9 fM, respectively. Finally, this proposed strategy was employed to simultaneously detect double miRNAs in practical applicability.

拓展阅读：

云南师范大学胡蓉团队在美国食品化学《FOOD CHEMISTRY》发表最新研究成果

云师大化工学院胡蓉博士最新研究成果在ACS Applied Materials & Interfaces上刊登

云师大胡蓉博士在《Biosensors and Bioelectronics》上刊文

云南师范大学生物电分析领域中青年学者之 胡蓉

 胡蓉教授介绍

 https://hgxy.ynnu.edu.cn/info/1093/1954.htm

杨通介绍                                                                        

杨通                                                                    

男，1990年5月生，汉族，中共党员，博士研究生，分析化学专业，硕士研究生导师，云南省“兴滇英才支持计划”——青年人才

邮箱：yangtong2018@ynnu.edu.cn; yt09132149@163.com

主要研究方向

研究方向：化学与生物传感、纳米分析化学、功能性核酸传感技术、光谱分析及电分析化学

研究专长：功能性电纺纳米结构、多孔框架纳米结构的制备及其在可视化、光电或光热生化传感中的应用

研究业绩：近些年来，主持国家自然科学基金项目1项、云南省科技厅基础研究计划项目2项、云南省教育厅科研项目1项。以第一作者和通讯作者身份，已在Anal. Chem.、Biosens. Bioelectron.、Sens. Actuators, B、Food Chem.、TrAC, Trends Anal. Chem.、ACS Appl. Mater. Interfaces、Nanoscale等分析化学、材料化学相关领域期刊，共发表SCI论文、国内核心期刊论文20余篇。授权国家发明专利1项。

主要招生专业

分析化学专业

教育与工作经历

2018年09月～至今，云南师范大学 化学化工学院 应用化学系

2019年06月～2021年05月，云南省德宏州梁河县平山乡 驻村参与国家脱贫攻坚任务

2013年09月～2018年06月，西南大学 药学院 分析化学专业 博士/研究生（硕博连读）

2009年09月～2013年06月，曲靖师范学院 化学化工学院 应用化学专业 学士/本科

获奖成果和荣誉称号

1. 杨通 云南省“兴滇英才支持计划”——青年人才，2019年

2. 2022年指导马璐瑶等本科生参加“微瑞杯”第三届全国大学生化学实验创新设计大赛荣获西南赛区一等奖、全国总决赛一等奖（国家级A类赛事）

3. 2022年指导周爱齐等本科生参加第八届云南省“互联网+”大学生创新创业大赛荣获铜奖

主持科研项目

1. 国家自然科学基金项目青年基金项目，基于电纺纤维固相传感平台的肿瘤标志物可视化分析方法与技术，项目批准号：21904114，起止年限：2020/01—2022/12。

2. 云南省基础研究计划项目面上项目，基于DNA纳米机器的功能性电纺纤维生物传感膜的分析方法与技术，项目批准号：202201AT070028，起止年限：2022/06—2025/05。

3. 云南省基础研究计划项目青年项目，静电纺纳米纤维传感界面的功能性调控及其在生物检测中的应用，项目批准号：202001AU070067，起止年限：2020/06—2023/05。

4. 云南省教育厅科学研究项目，荧光电纺纤维固相膜传感平台在生化分析中的应用，项目批准号：2019J0066，起止年限：2019/06—2020/05

代表性著作、论文、专利等

论文

1. Tong Yang; Chun Mei Li; Jia Hui He; Bin Chen; Yuan Fang Li; Cheng Zhi Huang*. Ratiometrically Fluorescent Electrospun Nanofibrous Film as a Cu²⁺-Mediated Solid-Phase Immunoassay Platform for Biomarkers. Anal. Chem. 2018, 90, 9966-9974. (中国科学院分区：一区，IF₂₀₂₂=8.008)

2. Mei Li¹; De Yan Li¹; Zi Ying Li; Rong Hu; Yun Hui Yang*; Tong Yang*. A Visual Peroxidase Mimicking Aptasensor Based on Pt Nanoparticles-Loaded on Iron Metal Organic Gel for Fumonisin B1 Analysis in Corn Meal. Biosens. Bioelectron. 2022, 209, 114241. (中国科学院分区：一区，IF₂₀₂₂=12.545)

3. De Yan Li; Zi Ying Li; Lei Han; Shuang Meng; Rong Hu; Yun Hui Yang; Tong Yang*. Target-Induced Tripedal G-Quadruplex DNAzyme for Multicolor Visual Point-of-Care Testing of Biomarkers Using Au Nanorods-Decorated Electrospun Nanofibrous Films. Sens. Actuators, B 2022, 371, 132510. (中国科学院分区：一区，IF₂₀₂₂=9.221)

4. Tong Yang*; Lei Zhan.; Cheng Zhi Huang**. Recent Insights into Functionalized Electrospun Nanofibrous Films for Chemo-/Bio-Sensors. TrAC, Trends Anal. Chem. 2020, 124, 115813. (中国科学院分区：一区，IF₂₀₂₂=14.908)

5. Yan Guan; Peng Bin Si; Tong Yang*; Yuan Wu*; Yun Hui Yang; Rong Hu*. A Novel Method for Detection of Ochratoxin A in Foods—Co-MOFs Based Dual Signal Ratiometric Electrochemical Aptamer Sensor Coupled with DNA Walker. Food Chem. 2023, 403, 134316. (中国科学院分区：一区，IF₂₀₂₂=9.231)

6. Zi Ying Li; De Yan Li; Long Huang; Rong Hu; Tong Yang*; Yun Hui Yang**. An Electrochemical Aptasensor Based on Intelligent Walking DNA Nanomachine with Cascade Signal Amplification Powered by Nuclease for Mucin 1 Assay. Anal. Chim. Acta 2022, 1214, 339964. (中国科学院分区：一区，IF₂₀₂₂=6.911)

7. Yan Guan; Fu Peng Wang; Zhi Xiong Chen; Yun Hui Yang; Tong Yang*; Rong Hu**. Ratiometrically Homogeneous Electrochemical Biosensor Based on the Signal Amplified Strategy of Dual DNA Nanomachines for MicroRNA Analysis. Talanta 2023, 254, 124191. (中国科学院分区：一区，IF₂₀₂₂=6.556)

8. Jia Li Liu¹; Yu Chan Ma¹; Tong Yang*; Rong Hu**; Yun Hui Yang***. A Single Nucleotide Polymorphism Electrochemical Sensor Based on DNA-Functionalized Cd-MOFs-74 as Cascade Signal Amplification Probes. Microchim. Acta 2021, 188(8), 266. (中国科学院分区：二区，IF₂₀₂₂=6.408)

9. Tong Yang; Peng Hou; Lin Ling Zheng.; Lei Zhan; Peng Fei Gao; Yuan Fang Li;* Cheng Zhi Huang*. Surface-Engineered Quantum Dots/Electrospun Nanofibers as A Networked Fluorescent Aptasensing Platform toward Biomarkers. Nanoscale 2017, 9, 17020-17028. (中国科学院分区：二区，IF₂₀₂₂=8.307)

10. Tong Yang; Jun Ma; Shu Jun Zhen;* Cheng Zhi Huang*. Electrostatic Assembly of Well-Dispersed AgNPs on the Surface of Electrospun Nanofibers as Highly Active SERS Substrates for Wide-Range pH Sensing. ACS Appl. Mater.Interfaces  2016, 8, 

14802-14811. (中国科学院分区：二区，IF₂₀₂₂=10.383)

11. Tong Yang; Hong Yan Zou; Cheng Zhi Huang*. Synergetic Catalytic Effect of Cu_2–xSe Nanoparticles and Reduced Graphene Oxide Coembedded in Electrospun Nanofibers for the Reduction of a Typical Refractory Organic Compound. ACS Appl. Mater. Interfaces 2015, 7, 15447-15457. (中国科学院分区：二区，IF₂₀₂₂=10.383)

12. Tong Yang; Hui Yang; Shu Jun Zhen;* Cheng Zhi Huang*. Hydrogen-Bond-Mediated in Situ Fabrication of AgNPs/Agar/PAN Electrospun Nanofibers as Reproducible SERS Substrates. ACS Appl. Mater. Interfaces 2015, 7, 1586-1594. (中国科学院分区：二区，IF₂₀₂₂=10.383)