近日，云南师范大学化学化工学院青年教师胡蓉团队在农林科学类一区TOP期刊以“A novel method for detection of ochratoxin A in foods-Co-MOFs based dual signal ratiometric electrochemical aptamer sensor coupled with DNA walker”发表最新研究成果，该成果报道了一种检测食品中基于Co-MOFs的双信号DNA walker耦合电化学传感检测赭曲霉毒素A（OchratoxinA）的新方法。

Highlights

• A DNA walker-based electrochemical ratiometric platform was developed.

• These dual signal ratiometric strategy overcome the changes of a variety of factors.

• A detection limit of 0.31 fg/mL for ochratoxin A (OTA) was achieved.

• The method was applied for determining OTA content in red wine samples.

Abstract

Ochratoxin A (OTA) is a naturally occurring mycotoxin that poses serious threats, such as kidney damage, to human health. Therefore, we developed a DNA walker-based dual-signal electrochemical ratiometric platform for OTA detection, which could overlook the variations in environmental and instrumental factors and DNA load densities. Cobalt metal–organic frameworks (Co-MOFs) and toluidine blue were used as the electrochemical signal tag and internal reference probe, respectively. In the presence of OTA, this developed machine resulted in the DNA labelled-Co-MOFs far away from the electrode. Thus, Co-MOFs signal at −1.18 V decreased, while toluidine blue at −0.28 V increased. This proposed strategy has displayed superior sensitivity (limit of detection = 0.31 fg/mL, linear range = 1–50 ng/mL) and high reproducibility. The sensor was also applied for determining OTA content in red wine samples and the results were comparable to those of commercial enzyme-linked immunoassay kits with satisfactory results.

全文链接：
https://www.sciencedirect.com/science/article/pii/S0308814622022786?via%3Dihub

据悉，

胡蓉，女，博士后，现就职于云南师范大学化学化工学院生物电分析课题组。

教育背景：

湖南大学博士，导师：张晓兵

佛罗里达州立大学博士后，导师：谭蔚泓，

代表著作：

1、DLISA:A DNAzyme-Based ELISA for Protein Enzyme-Free Immunoassay ofMultiple Analytes

作者: Hu, Rong; Liu, Tao; Zhang, Xiao-Bing;等.

ANALYTICALCHEMISTRY  卷: 87  期: 15  页: 7746-7753  出版年: AUG4 2015

2、DNANanoflowers for Multiplexed Cellular Imaging and Traceable TargetedDrug Delivery

作者: Hu, Rong; Zhang, Xiaobing; Zhao,Zilong; 等.

ANGEWANDTE CHEMIE-INTERNATIONALEDITION  卷: 53  期: 23  页: 5821-5826  出版年: JUN2014

3、MulticolorFluorescent Biosensor for Multiplexed Detection of DNA

作者: Hu, Rong; Liu, Tao; Zhang, Xiao-Bing;等.

ANALYTICALCHEMISTRY  卷: 86  期: 10  页: 5009-5016  出版年: MAY20 2014

4、Double-strandDNA-templated formation of Copper nanoparticles as fluorescentprobe for label free nuclease enzyme detection

作者: Hu, Rong; Liu, Ya-Ru; Kong, Rong-Mei;等.

BIOSENSORS &BIOELECTRONICS  卷: 42  页: 31-35  出版年: APR15 2013

5、Anefficient fluorescent sensing platform for biomolecules based onfenton reaction triggered molecular beacon cleavagestrategy

作者: Hu, Rong; Liu, Ya-Ru; Zhang, Xiao-Bing;等.

BIOSENSORS &BIOELECTRONICS  卷: 41  页: 442-445  出版年: MAR15 2013

6、Aproximity-dependent surface hybridization strategy for constructingan efficient signal-on electrochemical DNAzyme sensingsystem

作者: Hu, Rong; Fu, Ting; Zhang, Xiao-Bing;等.

CHEMICALCOMMUNICATIONS  卷: 48  期: 76  页: 9507-9509  出版年: 2012

7、Nucleicacid-functionalized nanomaterials for bioimagingapplications

作者: Hu, Rong; Zhang, Xiao-Bing; Kong,Rong-Mei; 等.

JOURNAL OF MATERIALSCHEMISTRY  卷: 21  期: 41  页: 16323-16334  出版年: 2011

8、Robustelectrochemical system for screening single nucleotidepolymorphisms

作者: Hu, Rong; Wu, Zai-Sheng; Zhang,Songbai; 等.

CHEMICALCOMMUNICATIONS  卷: 47  期: 4  页: 1294-1296  出版年: 2011

http://weibo.com/p/2304181320312830102xcgo

9.Bifunctional MOFs-Based Ratiometric Electrochemical Sensor for Multiplex Heavy Metal Ions

https://blog.sciencenet.cn/blog-454141-1030848.html

10.A Universal Aptameric Biosensor: Multiplexed Detection of Small Analytes via Aggregated Perylene-based Broad-Spectrum Quencher

https://blog.sciencenet.cn/blog-454141-1246974.html