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Li, Mei; Li, De Yan; Li, Zi Ying; Hu, Rong; Yang, Yun Hui; Yang, TongBiosensors & bioelectronics
Journal Article
A visual colorimetric aptasensor combining platinum nanoparticles (Pt NPs) loaded on Fe-based metal organic gel (Fe-MOG) as peroxidase mimics with magnetic separation technique was designed for the detection of fumonisin B1 (FB1). Interestingly, based on the inherent properties of Fe-MOG such as porous nanostructures, abundant Fe2+/Fe3+ active metal centers and synergetic effect between Pt NPs and Fe-MOG, Pt NPs/Fe-MOG composites can be employed as excellent peroxidase mimetic enzyme to oxidize 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. This process was accompanied by a color variation of the solution from colorless to blue. Kinetic analysis showed that the Pt NPs/Fe-MOG composites make an effective peroxidase mimic with low Michaelis constant (Km), high substrate binding affinity and rapid catalytic velocity (Vm). Furthermore, the Pt NPs/Fe-MOG based colorimetric aptasensor was used to detect FB1. The prepared Pt NPs/Fe-MOG achieved lower detection limit (2.7pg/mL, 3sigma/k) with a wide linear range of 0.01-2000.0ng/mL. Finally, as a proof of concept of the rapidity and the simplicity of this colorimetric aptasensor, Pt NPs/Fe-MOG was used for the detection of FB1 in real corn samples.
Copyright © 2022 Elsevier B.V. All rights reserved.
AptasensorColorimetric analysisCorn sampleFumonisin B(1) (FB(1))Metal-organic gels (MOGs)Peroxidase mimicking
College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, PR China.College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, PR China. Electronic address: yyhui2002@aliyun.com.College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, PR China. Electronic address: yt09132149@163.com.
Li, Mei; Li, De Yan; (...); Yang, Tong2022-Apr-05 | Biosensors & bioelectronics 209 , pp.114241
A visual colorimetric aptasensor combining platinum nanoparticles (Pt NPs) loaded on Fe-based metal organic gel (Fe-MOG) as peroxidase mimics with magnetic separation technique was designed for the detection of fumonisin B1 (FB1). Interestingly, based on the inherent properties of Fe-MOG such as porous nanostructures, abundant Fe2+/Fe3+ active metal centers and synergetic effect between Pt NPs
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