病毒的感染性和抗原性分析、病毒性疫苗的免疫原性分析和抗病毒药物的筛选等工作长期依赖于活病毒的分离和培养。然而，有些病毒难于大规模培养（如HPV等）,有些病毒培养依赖于安全等级较高的实验室（BSL-3或BSL-4），有些新突发传染病毒或新的变异株不易获得，这些限制了病毒学的研究和相应防控产品的开发。假病毒技术的出现解决了上述问题，已广泛用于病毒生物学特性研究和抗病毒产品开发与评价。特别是在新冠肺炎疫情期间，针对新型冠状病毒（SARS-CoV-2）的假病毒在世界范围内得到了广泛应用，已成为研究新型冠状病毒的有力工具。

    假病毒(Pseudovirus)的表面蛋白与活病毒具有相似的结构，并能介导假病毒进入易感细胞。假病毒没有病毒的完整基因组，只在易感细胞内进行一轮感染，与活病毒相比，基于假病毒的分析具有许多优点，包括：基于靶蛋白序列易于构建，不需要分离培养活病毒；假病毒只能进行一轮复制，安全性高，不需要高等级生物安全实验室；含有报告基因，易于定量分析；与活病毒蛋白结构类似，可模拟其真实功能，且易于操作。

    由中国医学科学院医学生物学研究所王佑春教授主编的《Pseudotyped Virus》（《假病毒》）已由Springer出版社出版。该专著由来自国内外多个假病毒研究团队的专家共同编撰，从假病毒的概念、构建、方法的开发和标准化、应用等方面对假病毒技术进行了系统总结，同时针对引起人类传染病的主要病毒种类，分章节进行了假病毒技术开发和应用的系统论述。该专著既对已有假病毒技术的开发和应用进行了总结，也对不同假病毒系统特点和不同病毒种类特征进行了分析归纳，对未来基于新病毒的假病毒技术开发具有重要的指导意义。

Pseudotyped Virus专著PDF下载链接：https://link.springer.com/book/10.1007/978-981-99-0113-5 

电子书链接 DOI: https://doi.org/10.1007/978-981-99-0113-5 

eBook ISBN: 978-981-99-0113-5

Published: 15 March 2023

书籍简介：This book intends to report the new progress of pseudotyped viruses, including the construction of pseudotyped viruses with different strategies or vectors for most important viruses. Especially for emerging viruses, optimization of the condition and parameters for assay development based on the pseudotyped viruses and widely application as surrogate of authentic virus to study the biological functions of virus, detection of neutralizing antibody, screening viral entry inhibiters, and others. It includes most pseudotyped viruses that have the protein of the target virus on the surface of the parent virus with incomplete genome. The book is likely to be of interest to all researchers in the field of virology, vaccine, and anti-viral drug development and evaluation.

Pseudotyped viruses are widely used as a surrogate of authentic viruses for emerging viruses.

Pseudotyped viruses must be used to evaluate the anti-viral products of viruses which cannot be cultured.

Includes construction, assay development, and application of pseudotyped viruses.

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 1407)

目录：

Pseudotyped Viruses

Youchun Wang, Zehua Zhou, Xi Wu, Tao Li, Jiajing Wu, Meina Cai et al.

Pages 1-27

Assays Based on Pseudotyped Viruses

Jianhui Nie, Xueling Wu, Youchun Wang

Pages 29-44

Application of Pseudotyped Viruses

Qianqian Cui, Weijin Huang

Pages 45-60

Pseudotyped Viruses for Retroviruses

Magan Solomon, Chen Liang

Pages 61-84

Pseudotyped Virus for Papillomavirus

Xueling Wu, Jianhui Nie, Youchun Wang

Pages 85-103

Pseudotyped Viruses for Marburgvirus and Ebolavirus

Li Zhang, Shou Liu, Youchun Wang

Pages 105-132

Pseudotyped Viruses for Coronaviruses

Meiyu Wang, Jianhui Nie, Youchun Wang

Pages 133-151

Pseudotyped Viruses for Influenza

Joanne Marie M. Del Rosario, Kelly A. S. da Costa, Nigel J. Temperton

Pages 153-173

Pseudotyped Virus for Henipavirus

Tao Li, Ziteng Liang, Weijin Huang, Youchun Wang

Pages 175-190

Pseudotyped Viruses for Lyssavirus

Wenbo Wang, Caifeng Long, Lan Wang, Youchun Wang

Pages 191-208

Pseudotyped Viruses for Enterovirus

Xing Wu, Lisha Cui, Yu Bai, Lianlian Bian, Zhenglun Liang

Pages 209-228

Pseudotyped Viruses for Orthohantavirus

Tingting Ning, Weijin Huang, Li Min, Yi Yang, Si Liu, Junxuan Xu et al.

Pages 229-252

Pseudotyped Viruses for Phlebovirus

Jiajing Wu, Weijin Huang, Youchun Wang

Pages 253-264

Pseudotyped Virus for Bandavirus

Ruifeng Chen, Weijing Huang, Youchun Wang

Pages 265-277

Pseudotyped Viruses for Mammarenavirus

Qianqian Li, Weijing Huang, Youchun Wang

Pages 279-297

Pseudotyped Viruses for the Alphavirus Chikungunya Virus

Jiajing Wu, Weijin Huang, Youchun Wang

Pages 299-312

Pseudotyped Virus for Flaviviridae

Leiliang Zhang, Xiao Wang, Annan Ming, Wenjie Tan

Pages 313-327

Replicating-Competent VSV-Vectored Pseudotyped Viruses

Fei Yuan, Aihua Zheng

Pages 329-348