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保护碱基加多少

已有 30534 次阅读 2011-12-14 13:38 |系统分类:科研笔记

很多版本，先留下参考：

新版本的只限制碱基数量：

| A | B | E | H | K | M | N | P | S | X |

Enzyme	Base pairs from End	%Cleavage Efficiency	Vector	Initial Cut
AatII	3 2 1	88 100 95	LITMUS 29 LITMUS 28 LITMUS 29	NcoI NcoI PinAI
Acc65I	2 1	99 75	LITMUS 29 pNEB193	SpeI SacI
AflII	1	13	LITMUS 29	StuI
AgeI	1 1	100 100	LITMUS 29 LITMUS 29	XbaI AatII
ApaI	2	100	LITMUS 38	SpeI
AscI	1	97	pNEB193	BamHI
AvrII	1	100	LITMUS 29	SacI
BamHI	1	97	LITMUS 29	HindIII
BglII	3	100	LITMUS 29	NsiI
BsiWI	2	100	LITMUS 29	BssHII
BspEI	2 1	100 8	LITMUS 39 LITMUS 38	BsrGI BsrGI
BsrGI	2 1	99 88	LITMUS 39 LITMUS 38	SphI BspEI
BssHII	2	100	LITMUS 29	BsiWI
EagI	2	100	LITMUS 39	NheI
EcoRI	1 1 1	100 88 100	LITMUS 29 LITMUS 29 LITMUS 39	XhoI PstI NheI
EcoRV	1	100	LITMUS 29	PstI
HindIII	3 2 1	90 91 0	LITMUS 29 LITMUS 28 LITMUS 29	NcoI NcoI BamHI
KasI	2 1	97 93	LITMUS 38 LITMUS 38	NgoMIV HindIII
KpnI	2 2 1	100 100 99	LITMUS 29 LITMUS 29 pNEB193	SpeI SacI SacI
MluI	2	99	LITMUS 39	EagI
MunI	2	100	LITMUS 39	NgoMIV
NcoI	2	100	LITMUS 28	HindIII
NgoMIV	2	100	LITMUS 39	MunI
NheI	1 2	100 82	LITMUS 39 LITMUS 39	EcoRI EagI
NotI	7 4 1	100 100 98	Bluescript SK- Bluescript SK- Bluescript SK-	SpeI KspI XbaI
NsiI	3 3 2	100 77 95	LITMUS 29 LITMUS 29 LITMUS 28	BssHII BglII BssHII
PacI	1	76	pNEB193	BamHI
PmeI	1	94	pNEB193	PstI
PstI	3 2 1	98 50 37	LITMUS 29 LITMUS 39 LITMUS 29	EcoRV HindIII EcoRI
SacI	1	99	LITMUS 29	AvrII
SalI	3 2 1	89 23 61	LITMUS 39 LITMUS 39 LITMUS 38	SpeI SphI SphI
SfiI^*	9 4 1	81 97 93	LITMUS 38 LITMUS 38 LITMUS 38	BamHI MluI EcoRI
SpeI	2 2	100 100	LITMUS 29 LITMUS 29	Acc65I KpnI
SphI	2 2 1	99 97 92	LITMUS 39 LITMUS 39 LITMUS 38	SalI BsrGI SalI
XbaI	1	99 94	LITMUS 29 LITMUS 29	AgeI PinAI
XhoI	1	97	LITMUS 29	EcoRI
XmaI	2 2	98 92	pNEB193 pNEB193	AscI BssHII

来源于：http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp

Enzyme	Bp from the recognition site to fragment end
Enzyme	1	2	3	4	5
AanI (PsiI)	0	0-20	20-50
AarI	20-50		50-100
AasI (DrdI)	50-100
AatII	0	0-20	20-50	50-100
Acc65I (Asp718I)	0-20	50-100
AdeI (DraIII)	50-100
AjiI (BmgBI)	50-100
AluI	0-20	20-50	50-100
Alw21I (BsiHKAI)	50-100
Alw26I	50-100
Alw44I	0	20-50	50-100
ApaI	50-100
BamHI	50-100
BauI (BssSI)	0-20	20-50	50-100
BclI	0	50-100
BcnI (NciI)	20-50		50-100
BcuI (SpeI)	50-100
BfiI (BmrI)	50-100
BfmI (SfcI)	50-100
BfuI (BciVI)	50-100
BglI	20-50	50-100
BglII	0	50-100
Bme1390I (ScrFI)	20-50		50-100
BoxI (PshAI)	0	50-100
BpiI (BbsI)	50-100
Bpu10I	20-50	50-100
Bpu1102I (BlpI)	50-100
BseDI (BsaJI)	0	50-100
BseGI (BtsCI)	50-100
BseJI (BsaBI)	0	50-100
BseLI (BslI)	0	50-100
BseMI (BsrDI)	0-20	50-100
BseMII (BspCNI)	50-100
BseNI (BsrI)	0	50-100
BseSI (Bme1580I)	50-100
BseXI (BbvI)	20-50	50-100
Bsh1236I (BstUI)	50-100
Bsh1285I (BsiEI)	0-20	50-100
BshNI (BanI)	50-100
BshTI (AgeI)	20-50	50-100
Bsp119I (BstBI)	50-100
Bsp120I (PspOMI)	20-50	50-100
Bsp1407I (BsrGI)	20-50	50-100
Bsp143I (Sau3AI)	50-100
Bsp68I (NruI)	0	50-100
BspLI (NlaIV)	50-100
BspOI (BmtI)	0	20-50	50-100
BspPI (AlwI)	0	50-100
BspTI (AflII)	0	0-20	50-100
Bst1107I (BstZ17I)	0-20	50-100
BstXI	0		50-100
Bsu15I (ClaI)	50-100
BsuRI (HaeIII)	0-20	20-50	50-100
BveI (BspMI)	0-20		50-100
CaiI (AlwNI)	0	0-20	50-100
Cfr10I (BsrFI)	20-50	50-100
Cfr13I (Sau96I)	50-100
Cfr42I (SacII)	50-100
Cfr9I (XmaI)	20-50	50-100
CfrI (EaeI)	0	50-100
CpoI (RsrII)	50-100
CseI (HgaI)	50-100
Csp6I (CviQI)	50-100
DpnI	50-100
DraI	0	0-20	50-100
Eam1104I (EarI)	0	50-100
Eam1105I (AhdI)	0	50-100
Ecl136II (EcoICRI)	50-100
Eco105I (SnaBI)	20-50	50-100
Eco130I (StyI)	0		50-100
Eco147I (StuI)	0	50-100
Eco24I (BanII)	50-100
Eco31I (BsaI)	20-50		50-100
Eco32I (EcoRV)	20-50	50-100
Eco47I (AvaII)	50-100
Eco47III (AfeI)	0	0-20	50-100
Eco52I (EagI)	0-20	50-100
Eco57I (AcuI)	50-100
Eco57MI	50-100
Eco72I (PmlI)	0-20	50-100
Eco81I (Bsu36I)	50-100
Eco88I (AvaI)	50-100
Eco91I (BstEII)	20-50	50-100
EcoO109I (DraII)	50-100
EcoRI	50-100
EcoRII	0	0-20	20-50	50-100
EheI (SfoI)	20-50		50-100
Esp3I (BsmBI)	50-100
FaqI (BsmFI)	0-20	50-100
FspAI	0-20	20-50	50-100
FspBI (BfaI)	20-50	50-100
GsuI (BpmI)	50-100
HhaI	50-100
Hin1I (BsaHI)	50-100
Hin1II (NlaIII)	0	0-20	50-100
Hin6I (HinP1I)	0		0-20	50-100
HincII (HindII)	50-100
HindIII	0	0-20	50-100
HinfI	50-100
HpaII	0-20	50-100
HphI	0	50-100
Hpy8I (MjaIV)	0-20	50-100
HpyF10VI (MwoI)	50-100
HpyF3I (DdeI)	20-50	50-100
I-SceI	50-100
Kpn2I (BspEI)	0	50-100
KpnI	50-100
KspAI (HpaI)	20-50	50-100
LguI (SapI)	50-100
Lsp1109I (BbvI)	0-20	50-100
LweI (SfaNI)	20-50	50-100
MauBI	0	0-20		20-50
MbiI (BsrBI)	0	0-20	50-100
MboI	50-100
MboII	50-100
MlsI (MscI)	0-20	50-100
MluI	20-50		50-100
MnlI	0	50-100
Mph1103I (NsiI)	20-50	50-100
MreI (Sse232I)	0	20-50	50-100
MspI (HpaII)	0-20	50-100
MssI (PmeI)	20-50	50-100
MunI (MfeI)	20-50	50-100
Mva1269I (BsmI)	0	50-100
MvaI (BstNI)	0	20-50	50-100
NcoI	0	50-100
NdeI	0-20	20-50	50-100
NheI	0	20-50		50-100
NmuCI (Tsp45I)	20-50	50-100
NotI	20-50	50-100
NsbI (FspI)	0	0-20	50-100
OliI (AleI)	0-20	20-50	50-100
PacI	20-50	50-100
PaeI (SphI)	0	0-20		20-50	50-100
PagI (BspHI)	20-50	50-100
PasI	50-100
PauI (BssHII)	0	50-100
PdiI (NaeI)	0-20	50-100
PdmI (XmnI)	50-100
PfeI (TfiI)	50-100
Pfl23II (BsiWI)	0-20	20-50	50-100
PfoI	0	20-50	50-100
Ppu21I (BsaAI)	50-100
PscI (PciI)	0	50-100
Psp1406I (AclI)	0-20	50-100
Psp5II (PpuMI)	0	50-100
PstI	0-20	50-100
PsuI (BstYI)	0-20	50-100
PsyI (Tth111I)	0	50-100
PvuI	20-50		50-100
PvuII	50-100
RsaI	50-100
RseI (MslI)	0	0-20	20-50	50-100
SacI	50-100
SalI	20-50		50-100
SatI (Fnu4HI)	0	50-100
ScaI	0-20	50-100
SchI (MlyI)	50-100
SdaI (SbfI)	0-20	50-100
SduI (Bsp1286I)	50-100
SfaAI (AsiSI)	0	0-20		20-50
SfiI	50-100
SgrDI	0-20	20-50	50-100
SgsI (AscI)	50-100
SmaI	50-100
SmiI (SwaI)	0-20	50-100
SmoI (SmlI)	0	50-100
SmuI (FauI)	50-100
SsiI (AciI)	50-100
SspDI (KasI)	20-50		50-100
SspI	0-20	50-100
TaaI (HpyCH4III)	20-50	50-100
TaiI (MaeII)	50-100
TaqI	0	20-50		50-100
TasI (Tsp509I)	0	20-50	50-100
TatI	0-20		20-50	50-100
TauI	20-50	50-100
Tru1I (MseI)	0	0-20		50-100
TscAI (TspRI)	0	50-100
TsoI	20-50	50-100
Van91I (PflMI)	0	50-100
VspI (AseI)	50-100
XagI (EcoNI)	20-50	50-100
XapI (ApoI)	20-50		50-100
XbaI	20-50	50-100
XceI (NspI)	0	50-100
XhoI	0-20	50-100
XmaJI (AvrII)	50-100
XmiI (AccI)	50-100

Enzyme	Bp from the recognition site to fragment end
Enzyme	1	2	3	4	5
AanI (PsiI)	0	0-20	20-50
AarI	20-50		50-100
AasI (DrdI)	50-100
AatII	0	0-20	20-50	50-100
Acc65I (Asp718I)	0-20	50-100
AdeI (DraIII)	50-100
AjiI (BmgBI)	50-100
AluI	0-20	20-50	50-100
Alw21I (BsiHKAI)	50-100
Alw26I	50-100
Alw44I	0	20-50	50-100
ApaI	50-100
BamHI	50-100
BauI (BssSI)	0-20	20-50	50-100
BclI	0	50-100
BcnI (NciI)	20-50		50-100
BcuI (SpeI)	50-100
BfiI (BmrI)	50-100
BfmI (SfcI)	50-100
BfuI (BciVI)	50-100
BglI	20-50	50-100
BglII	0	50-100
Bme1390I (ScrFI)	20-50		50-100
BoxI (PshAI)	0	50-100
BpiI (BbsI)	50-100
Bpu10I	20-50	50-100
Bpu1102I (BlpI)	50-100
BseDI (BsaJI)	0	50-100
BseGI (BtsCI)	50-100
BseJI (BsaBI)	0	50-100
BseLI (BslI)	0	50-100
BseMI (BsrDI)	0-20	50-100
BseMII (BspCNI)	50-100
BseNI (BsrI)	0	50-100
BseSI (Bme1580I)	50-100
BseXI (BbvI)	20-50	50-100
Bsh1236I (BstUI)	50-100
Bsh1285I (BsiEI)	0-20	50-100
BshNI (BanI)	50-100
BshTI (AgeI)	20-50	50-100
Bsp119I (BstBI)	50-100
Bsp120I (PspOMI)	20-50	50-100
Bsp1407I (BsrGI)	20-50	50-100
Bsp143I (Sau3AI)	50-100
Bsp68I (NruI)	0	50-100
BspLI (NlaIV)	50-100
BspOI (BmtI)	0	20-50	50-100
BspPI (AlwI)	0	50-100
BspTI (AflII)	0	0-20	50-100
Bst1107I (BstZ17I)	0-20	50-100
BstXI	0		50-100
Bsu15I (ClaI)	50-100
BsuRI (HaeIII)	0-20	20-50	50-100
BveI (BspMI)	0-20		50-100
CaiI (AlwNI)	0	0-20	50-100
Cfr10I (BsrFI)	20-50	50-100
Cfr13I (Sau96I)	50-100
Cfr42I (SacII)	50-100
Cfr9I (XmaI)	20-50	50-100
CfrI (EaeI)	0	50-100
CpoI (RsrII)	50-100
CseI (HgaI)	50-100
Csp6I (CviQI)	50-100
DpnI	50-100
DraI	0	0-20	50-100
Eam1104I (EarI)	0	50-100
Eam1105I (AhdI)	0	50-100
Ecl136II (EcoICRI)	50-100
Eco105I (SnaBI)	20-50	50-100
Eco130I (StyI)	0		50-100
Eco147I (StuI)	0	50-100
Eco24I (BanII)	50-100
Eco31I (BsaI)	20-50		50-100
Eco32I (EcoRV)	20-50	50-100
Eco47I (AvaII)	50-100
Eco47III (AfeI)	0	0-20	50-100
Eco52I (EagI)	0-20	50-100
Eco57I (AcuI)	50-100
Eco57MI	50-100
Eco72I (PmlI)	0-20	50-100
Eco81I (Bsu36I)	50-100
Eco88I (AvaI)	50-100
Eco91I (BstEII)	20-50	50-100
EcoO109I (DraII)	50-100
EcoRI	50-100
EcoRII	0	0-20	20-50	50-100
EheI (SfoI)	20-50		50-100
Esp3I (BsmBI)	50-100
FaqI (BsmFI)	0-20	50-100
FspAI	0-20	20-50	50-100
FspBI (BfaI)	20-50	50-100
GsuI (BpmI)	50-100
HhaI	50-100
Hin1I (BsaHI)	50-100
Hin1II (NlaIII)	0	0-20	50-100
Hin6I (HinP1I)	0		0-20	50-100
HincII (HindII)	50-100
HindIII	0	0-20	50-100
HinfI	50-100
HpaII	0-20	50-100
HphI	0	50-100
Hpy8I (MjaIV)	0-20	50-100
HpyF10VI (MwoI)	50-100
HpyF3I (DdeI)	20-50	50-100
I-SceI	50-100
Kpn2I (BspEI)	0	50-100
KpnI	50-100
KspAI (HpaI)	20-50	50-100
LguI (SapI)	50-100
Lsp1109I (BbvI)	0-20	50-100
LweI (SfaNI)	20-50	50-100
MauBI	0	0-20		20-50
MbiI (BsrBI)	0	0-20	50-100
MboI	50-100
MboII	50-100
MlsI (MscI)	0-20	50-100
MluI	20-50		50-100
MnlI	0	50-100
Mph1103I (NsiI)	20-50	50-100
MreI (Sse232I)	0	20-50	50-100
MspI (HpaII)	0-20	50-100
MssI (PmeI)	20-50	50-100
MunI (MfeI)	20-50	50-100
Mva1269I (BsmI)	0	50-100
MvaI (BstNI)	0	20-50	50-100
NcoI	0	50-100
NdeI	0-20	20-50	50-100
NheI	0	20-50		50-100
NmuCI (Tsp45I)	20-50	50-100
NotI	20-50	50-100
NsbI (FspI)	0	0-20	50-100
OliI (AleI)	0-20	20-50	50-100
PacI	20-50	50-100
PaeI (SphI)	0	0-20		20-50	50-100
PagI (BspHI)	20-50	50-100
PasI	50-100
PauI (BssHII)	0	50-100
PdiI (NaeI)	0-20	50-100
PdmI (XmnI)	50-100
PfeI (TfiI)	50-100
Pfl23II (BsiWI)	0-20	20-50	50-100
PfoI	0	20-50	50-100
Ppu21I (BsaAI)	50-100
PscI (PciI)	0	50-100
Psp1406I (AclI)	0-20	50-100
Psp5II (PpuMI)	0	50-100
PstI	0-20	50-100
PsuI (BstYI)	0-20	50-100
PsyI (Tth111I)	0	50-100
PvuI	20-50		50-100
PvuII	50-100
RsaI	50-100
RseI (MslI)	0	0-20	20-50	50-100
SacI	50-100
SalI	20-50		50-100
SatI (Fnu4HI)	0	50-100
ScaI	0-20	50-100
SchI (MlyI)	50-100
SdaI (SbfI)	0-20	50-100
SduI (Bsp1286I)	50-100
SfaAI (AsiSI)	0	0-20		20-50
SfiI	50-100
SgrDI	0-20	20-50	50-100
SgsI (AscI)	50-100
SmaI	50-100
SmiI (SwaI)	0-20	50-100
SmoI (SmlI)	0	50-100
SmuI (FauI)	50-100
SsiI (AciI)	50-100
SspDI (KasI)	20-50		50-100
SspI	0-20	50-100
TaaI (HpyCH4III)	20-50	50-100
TaiI (MaeII)	50-100
TaqI	0	20-50		50-100
TasI (Tsp509I)	0	20-50	50-100
TatI	0-20		20-50	50-100
TauI	20-50	50-100
Tru1I (MseI)	0	0-20		50-100
TscAI (TspRI)	0	50-100
TsoI	20-50	50-100
Van91I (PflMI)	0	50-100
VspI (AseI)	50-100
XagI (EcoNI)	20-50	50-100
XapI (ApoI)	20-50		50-100
XbaI	20-50	50-100
XceI (NspI)	0	50-100
XhoI	0-20	50-100
XmaJI (AvrII)	50-100
XmiI (AccI)	50-100

from：http://www.fermentas.com/en/support/technical-reference/restriction-enzymes/cleavage-efficiency

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