Reviewer #1: I do not see the benefit of using this protocol over previously reported protocols as described in xxx There is no really a consistency report (the authors would need to extract several 100s samples and provide evidence of conistent amplification), whereas all tissue used is what I consider 'easy' - young leaves and petals. As a matter of fact, you can extract RNA from petals even with RNeasy. Not MBio quality by any means. Why not use old leaves and see the quality of amplification? I know for a fact that there are protocols out there that can do this very efficiently, it's just that the authors did not dig deep enough. 确实是没有提到那么多材料,要是提了那么多材料,我不会选这个期刊。
Reviewer #2,
This paper proposes a simple, rapid and specific xxxxx and this is a subject of potential interest. However, this paper presents several incorrections that you must correct for its publication in Molecular Biotecnology. For that reason I consider that this manuscript must be carefully adjusted according to the suggestions I make below.然后是要修改的地方。