最近日本全州广岛大学学者Shinya Kato等在Materials Science andEngineering: C发表论文Antioxidant activities of nano-bubblehydrogen-dissolved water assessed by ESR and 2,2’-bipyridyl methods。研究采用两种自由基检测方法，证明纳米气泡水制备氢气水抗氧化效果比普通的氢气水更明显。研究的水包括纯净水、自来水、普通商品氢气水、工业化氢气水和纳米气泡氢气水等类型。从氢气浓度分析看，纯净水、自来水几乎没有氢气，普通商品化氢气水氢气浓度非常低，这也符合日本市场特点，大部分氢水中氢气浓度非常低，本次研究中的氢水氢气浓度为0.075 ppm, ORP+ 49 mV，这种浓度大约为饱和浓度的1/20。研究中的工业化氢气水，我个人理解是刚刚生产出没有经过运输上市的氢水，这种氢水中氢气的浓度可以达到0.788 ppm, ORP− 614 mV)这是比较符合情况的，这种浓度大约相当于饱和度的50%。他们的主要研究目标，氢气纳米气泡水，氢气形成纳米气泡中54%大小小于717纳米（或者说是0.717微米），这是相对比较大的气泡，纳米气泡氢气水中氢气浓度稍微低于工业生产的氢气水。而国内的纳米氢气水生产的氢气水浓度远远高于日本这一文章所报道的数据。相信日本的工业技术应该不错，相信他们完全可以生产出更高浓度的氢气水，为什么会出现这种情况，我无法了解原因。

研究采用的抗氧化能力检测，一种是顺磁共振技术，这种技术是检测自由基的最高端技术，顺磁共振本质上是检测电子自旋产生的磁场，因为自由基存在不成对电子，所以可以产生信号，而传统的磁共振信号是核自旋形成的磁场。一般医院中的MRI原理都是检测的核磁共振信号，与顺磁共振不一样。另外一种2、2联吡啶氧化还原分光光度法，2、2联吡啶这是一种氧化还原指示剂，是一种白色或浅红色结晶性粉末，易溶于醇、醚、苯、三氯甲烷和石油醚。可溶于水，溶液遇亚铁盐则显红色。两种研究方法都显示，虽然纳米气泡氢气水中氢气的浓度比工业生产的氢水低，但是其抗羟基自由基的能力更高，这一研究说明，纳米气泡氢气水具有更理想的抗氧化效果，可能具有更好的商业前景。

本论文通信作者Nobuhiko Miwa彦美和，联系方法

E-mail:miwa@butsuryo.ac.jp,

TEL:+81-072-260-0088,

FAX:+81-072-260-0011

ShinyaKato, Daigo Matsuok, Nobuhiko Miw,

Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR a.pdf

Laboratoryof Cell-Death Control BioTechnology, Faculty of Life and EnvironmentalSciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara,Hiroshima 727–0023,Japan.

We prepared nano-bubble hydrogen-dissolvedwater (nano-H water) which contained hydrogen nano-bubblesof < 717-nm diameter for 54% of total bubbles. In DMPO-spin trapelectron spin resonance (ESR) method, the DMPO-OH : MnO ratio, being attributedto amounts of hydroxyl radicals (·OH), was 2.78 for pure water (dissolvedhydrogen [DH] = < 0.01 ppm, oxidation-reduction potential[ORP] = + 324 mV), 2.73 for tap water (0.01 ppm,+ 286 mV), 2.93 for commercially available hydrogen water(0.075 ppm, + 49 mV), and 2.66 for manufactured hydrogen water(0.788 ppm, − 614 mV), whereas the nano-H water (0.678 ppm,− 644 mV) exhibited 2.05, showing the superiority of nano-H water toother types of hydrogen water in terms of · OH-scavenging activity.Then, reduction activity of nano-H water was assessed spectrophotometrically by2,2’-bipyridyl method. Differential absorbance at 530 nm was in the order:0.018 for pure water, 0.055 for tap water, 0.079 for nano-H water, 0.085 forcommercially available hydrogen water, and 0.090 for manufactured hydrogenwater, indicating a prominent reduction activity of hydrogen water and nano-Hwater against oxidation in ascorbate-coupled ferric ion-bipyridyl reaction.Thus, the nano-H water has an improved antioxidant activity as compared to hydrogenwater of the similar DH-level, indicating the more marked importance ofnano-bubbles rather than concentration of hydrogen in termsof · OH-scavenging.

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