http://blogs.nature.com/news/2014/06/genetic-tests-suggest-stap-stem-cells-never-existed.html

基因分析表明STAP干细胞从没有存在过

Genetic tests suggest STAPstem cells ‘never existed’

通过酸处理和挤压等简单应激处理，将成熟细胞诱导成胚胎干细胞，这一“新”成果在《自然》杂志上一发表，就引起国际干细胞学研究领域的轰动，更大的新闻是随后这些论文被人质疑存在图片造假和文字抄袭等学术不端嫌疑，并引起作者单位介入调查。这一新闻的起源是日本理化研究所发育研究中心负责人小保方最近发表的2篇论文。该研究所调查委员会很快认定小保方发表论文的一些问题属实，并确定她属于学术不端，上周小保方已经同意将其中关于STAP可分化为其他组织的论文撤稿，但拒绝撤回关于STAP制作方法的论文，她也一直坚信这种STAP细胞真实不虚。承认错误不等于否定科学发现。科学连续剧到了现在这个阶段，有一个很关键问题需要明确，到底STAP细胞是否属实？

今天日本新闻报道说，基因检测数据提示，STAP细胞从来不存在。

上个月论文共同作者之一山梨大学Teruhiko Wakayama教授，通过基因分析发现，一些STAP细胞系和论文描述的来源不符。结果显示这些细胞可能是污染了其他细胞。但Teruhiko Wakayama教授没有发现这些细胞存在污染问题。为确定他的判断，Wakayama将20种细胞送到一个独立研究机构进一步确认，当时他没有透漏具体的送检单位。根据日本媒体的最新报道，这些细胞送到理化研究所和那些发现论文错误的研究机构。检测结论是，所有STAP细胞都不符合论文声称的来源，说明所谓STAP细胞是子虚乌有。Wakayama说他将在最近的新闻发布会上公布详细结果。

STAP cells were claimed tobe made by exposing bodily cells to acid or subjecting them to physicalpressure. These cells take on characteristics of embryonic stem cells. Iffurther manipulated, they will also form self-renewing stem cell lines, calledSTAP stem cells, which share most properties of the embryonic stem cell lines.The Nature papers reported the creation of eight STAP stem cell lines. (Note:Nature’s news and comment team is editorially independent of its researcheditorial team.)

In March, one of the co-authorsof the STAP papers, Teruhiko Wakayama of Yamanashi University, did a simplegenetic analysis and found that some of the supposed STAP stem cell lines hehad produced outside of the experiments described in the papers did not matchthe strain of mouse from which they were supposed to have been derived. Thiswould mean that the cells came from a different mouse to that claimed,suggesting contamination. But he did not find a problem with the STAP stem celllines that were reported in the Nature papers. To verify his results, Wakayamasent some 20 stem cell lines, including samples of the eight reported in thepapers, to an independent, but unnamed, genetic analysis team for more precisetests.

 According to Japanese mediareports quoting “multiple sources”, the results of those tests have now beensent to RIKEN, Obokata’s employer and the institution that found her guilty ofmisconduct. They conclude that none of the STAP stem cell lines match theoriginal mouse strains from which they were supposedly taken, calling intoquestion whether the STAP phenomenon has ever been demonstrated. Wakayama sayshe will release detailed results at a press conference soon.

相关专题：小保方事件追踪
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