呼吸氢对多种器官缺血再灌注损伤的治疗作用已经有非常多的报道，但过去没有人采用双侧颈总动脉缺血再灌注脑损伤进行研究，来自日本国防医科大学神经外科的学者最近报道了呼吸氢对这类损伤的治疗作用的研究。模型采用双侧颈总动脉结扎，45分钟后恢复血流，并连续检测脑血流180分钟。研究动物选择雄性C57BL/6J小鼠，将动物分成假手术组、模型组和治疗组，治疗组动物呼吸1.3%氢气，手术（45分钟+180分钟）结束后，给动物连续呼吸3小时治疗气体，手术后第2和第3天同样呼吸3小时（每天一次3小时连续3天），分别对脑含水量、海马神经细胞病理学改变和免疫组织化学检测氧化指标（8羟基脱氧鸟嘌呤）。研究结果发现，和对照组相比，呼吸氢组动物上述指标有明显改变。结果提示，氢呼吸对双侧颈总动脉缺血再灌注脑损伤具有治疗作用。

http://link.springer.com/chapter/10.1007%2F978-3-7091-1434-6_10#

The Effect of Hydrogen Gas on a Mouse Bilateral Common Carotid Artery Occlusion

In recent studies, molecular hydrogen selectively reduced the levels of hydroxyl radicals in vitro and exerted a therapeutic anti-oxidant activity in a rat middle cerebral artery occlusion model. The aim of this study was to investigate the effect of hydrogen gas on a mouse bilateral common carotid artery occlusion (BCCAO) model. Male C57BL/6J mice were subjected to transient BCCAO with a nontraumatic aneurysm clip. The mice were divided into three groups: sham, BCCAO, and BCCAO treated with 1.3 % hydrogen gas. Cerebral blood flow (CBF) in the cortex was measured sequentially for both hemispheres with a non-­invasive and noncontact laser Doppler blood perfusion imager during the procedure. Vital signs were also recorded. Oxidative stress evaluated by measuring the level of 8-hydroxy-2’-deoxyguanosine (8-OHdG), neuronal injury in the hippocampal CA1 sector, and brain water content were assessed 24 h after ischemia. The hydrogen gas treatment had no significant effect on vital signs or CBF values. However, the reduction of the expression of 8-OHdG, the decrease in the neuronal injury in the hippocampal CA1 sector, and the attenuation in brain water content were observed in hydrogen-treated mice. In conclusion, hydrogen gas might be effective in a mouse BCCAO model.