刚看到一篇文章是研究经过皮肤摄取二氧化碳的研究，根据这个思路，氢气、一氧化碳、氧气等是否也可以经过皮肤来摄取，如果设法提高摄取速度，是否可以作为局部疾病治疗的手段。

A Novel System for Transcutaneous Application of Carbon Dioxide Causing an “Artificial Bohr Effect” in the Human Body

Background

Carbon dioxide (CO₂) therapy refers to the transcutaneous administration of CO₂ for therapeutic purposes. This effect has been explained by an increase in the pressure of O₂ in tissues known as the Bohr effect. However, there have been no reports investigating the oxygen dissociation of haemoglobin (Hb) during transcutaneous application of CO₂ in vivo. In this study, we investigate whether the Bohr effect is caused by transcutaneous application of CO2 in human living body.

Methods

We used a novel system for transcutaneous application of CO₂ using pure CO₂ gas, hydrogel, and a plastic adaptor. The validity of the CO₂ hydrogel was confirmed in vitro using a measuring device for transcutaneous CO₂ absorption using rat skin. Next, we measured the pH change in the human triceps surae muscle during transcutaneous application of CO₂ using phosphorus-31 magnetic resonance spectroscopy (³¹P-MRS) in vivo. In addition, oxy- and deoxy-Hb concentrations were measured with near-infrared spectroscopy in the human arm with occulted blood flow to investigate O2 dissociation from Hb caused by transcutaneous application of CO₂.

Results

The rat skin experiment showed that CO₂ hydrogel enhanced CO₂ gas permeation through the rat skin. The intracellular pH of the triceps surae muscle decreased significantly 10 min. after transcutaneous application of CO₂. The NIRS data show the oxy-Hb concentration decreased significantly 4 min. after CO₂ application, and deoxy-Hb concentration increased significantly 2 min. after CO₂ application in the CO₂-applied group compared to the control group. Oxy-Hb concentration significantly decreased while deoxy-Hb concentration significantly increased after transcutaneous CO₂ application.

Conclusions

Our novel transcutaneous CO₂ application facilitated an O₂ dissociation from Hb in the human body, thus providing evidence of the Bohr effect in vivo.

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