2月前我国学者刚刚有关于睾丸缺血再灌注损伤的文章被接受，最近，来自韩国建国大学兽医学院的另一篇同类文章发表在Journal of Pediatric Surgery。文章题目为Inhaled hydrogengas therapy for prevention of testicular ischemia/reperfusion injury in rats。和我国学者采用注射氢气盐水不同的是采用呼吸氢气。模型采用单侧睾丸缺血5小时，分别观察呼吸2%氢气30、60、120分钟后，形态学和生物化学指标，证明呼吸氢气可以剂量依赖性减少细胞凋亡数量和氧化损伤程度。结果表明，呼吸氢气可以通过抗凋亡和抗氧化作用治疗睾丸缺血再灌注损伤。

· http://dx.doi.org/10.1016/j.jpedsurg.2011.09.035, How to Cite or Link Using DOI

Abstract

Purpose

This study evaluated whether 2% hydrogen (H₂) gas therapy protects against testicular ischemia/reperfusion injury which results in increased formation of reactive oxygen species and/or reactive nitrogen species, leading to testicular apoptosis and impaired spermatogenesis.

Methods

Pubertal six-week-old Spraque-Dawley rats were assigned to 5 groups (10 animals/group) as follows: group A was a sham operated group; groups B, C, D, and E underwent 5 hours of left testicular ischemia followed by 0, 30, 60, and 120 minutes of 2% H₂gas therapy, respectively. Histological analysis was performed to verify structure and morphology of the testes and to investigate Johnsen scores, mean seminiferous tubule diameter, and the number of germ cell layers to classify spermatogenesis. Germ cell apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling assay and Bax/Bcl-2 ratio real-time polymerase chain reaction. We also investigated malondialdehyde levels as an indicator of lipid peroxidation.

Results

Compared to the sham group (A), germ cell apoptosis and lipid peroxidation in the ischemia group (B) were significantly increased with abnormal morphology and impaired spermatogenesis. In contrast, amelioration of testicular damages was evident in the H₂ therapy groups (C, D, and E).

Conclusions

Our results showed that inhalation of 2% H₂gas may be a promising therapy with anti-apoptotic and anti-oxidant properties in cases of testicular ischemia/reperfusion injury.

Figures and tables from this article:

Fig. 1.. (1) Detection of TUNEL-positive apoptotic cells in left testis from five different groups (original magnification ×400). (A) Sham group has few apoptotic cells. (B and C) The number of apoptotic cells increases significantly in groups B and C. (D and E) The number of apoptotic cells decreases significantly in groups D and E showing morphology similar to that of normal testis. (2) The number of apoptotic cell per tubule in each group, AI-1 and AI-2 are significantly increased in groups B and C, compared to the sham group, and they are significantly decreased in group E, compared to group B. ⁎P < .05 vs group A; #P < .05 vs group B. AI-1(apoptotic index 1), the number of apoptotic cells per 100 tubules; AI-2(apoptotic index 2), the number of positive tubules per 100 tubules. Groups: A, control; B, IR; C, IR + 30 minutes 2% H2; D, IR + 60 minutes 2% H2; E, IR + 120 minutes 2% H2.

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Fig. 2.. Effect of hydrogen (H2) gas on malondialdehyde (MDA) levels in rat testicular tissue in each group. Data are mean ± SD. MDA values are expressed as micromoles of MDA per mg of protein (μmol/mg protein) ⁎P < .05 vs group A; #P < .05 vs group B; Groups: A, control; B, IR; C, IR + 30 minutes 2% H2; D, IR + 60 minutes 2% H2; E, IR + 120 minutes 2% H2.