Hydrogen-Rich Saline Attenuates Lung Ischemia-Reperfusion Injury in Rabbits.pdf

四川大学华西医院麻醉系最近在《外科研究杂志》上发表了氢气治疗肺缺血再灌注损伤的文章。该实验室是国际上最早开展氢气研究的单位之一，早在2008年就曾经在国际麻醉会议上率先报道了呼吸氢气对肾脏缺血再灌注损伤，虽然只有肾脏功能改善和基本的病理学研究，但仍是非常值得尽快发表的，当时国际上氢气的文章总共不到10篇，随便作一点工作就可以发，而且将会有许多引用。

这一研究是一个硕士研究生的毕业论文，不知道什么原因后来一直没有最后在国际学术杂志上发表，好像国内杂志也没有见到该报道，后来长海医院麻醉科报道了用氢气生理盐水治疗肾脏缺血的研究，日本学者也有类似报道，也就是说08年他们关于肾脏缺血的研究已经被别人抢先报道，基本失去发表的价值，这是非常可惜的事情。

虽然氢气在治疗各类器官缺血再灌注的研究中已经有许多报道，例如心脏、肝脏、脑、肾脏等，但肺的缺血再灌注损伤，显然有器官特异性，特别是炎症反应方面与其他器官有比较明显的区别。本研究采用兔肺脏缺血再灌注模型，用氢气饱和生理盐水治疗，结果发现氢气生理盐水可以改善模型动物肺功能，提高抗氧化酶活性，减少氧化损伤，组织学改变也出现相应改善。

Hydrogen -Rich Saline Attenuates Lung Ischemia-Reperfusion Injury in Rabbits

Hui Li M.D.^∗, Ronghua Zhou M.D.^∗, Jin Liu M.D.^{∗,  ,}  , Qian Li M.D.^∗, Jingyu Zhang M.D.^∗, Jinglan Mu M.D.^∗, Xuejun Sun M.D., Ph.D.^{†,  ,} 

Received 3 May 2011; Accepted 4 October 2011. Available online 25 October 2011.

Background

Hydrogen gas , an antioxidant agent, was found to protect against cerebral and myocardial ischemia-reperfusion (I/R) injury. In the present study, we investigated the effect of hydrogen-rich saline (HRS) on the I/R-induced lung injury.

Methods

Left lung of male New Zealand White rabbits rendered normothermic ischemia for 60 min and reperfused for up to 240 min. Treated animals received intraperitoneal injection of 5 mL/kg HRS or the same volume of normal saline 10 min before the start of reperfusion. Blood and lung tissue samples were obtained for blood gas and biochemical analyses. The tissues obtained from lower lobe of left lung were used for histologic examination.

Results

After 240 min of reperfusion, intraperitoneal administration of HRS increased PaO2/FiO2 ratio and superoxide dismutase activities, and decreased malondialdehyde contents, proinflammatory cytokines expression, and myeloperoxide activities, along with reduced wet/dry ratio and histologic injury scores (P < 0.05 versus I/R group).

Conclusions

These results suggest that intraperitoneal administration of HRS before reperfusion protects the lung from I/R injury. The protective effect seems to be closely related to regulating oxidative damage and antioxidant enzyme activities and neutrophil infiltration.

Loader.rt("abs_end"); Loader.feature('lp_embed').qCode("loadEmbedContent(EMBED_APC, 'embedAPCModule');") Key Words: hydrogen; lung; ischemia-reperfusion

Article Outline

·         Introduction

·         Materials and Methods

·          

o    Animal Preparation

o    Drug Preparation

o    Experimental Protocol

o    Blood Gas Analyses

o    Pulmonary Edema

o    Oxidative Stress and Myeloperoxidase Activities Assays

o    Proinflammatory Cytokines Determination in Lung Tissues

o    Histologic Examination

o    Statistical Analyses

·         Results

·          

o    Pulmonary Gas Exchange

o    Pulmonary Edema

o    Oxidative Stress

o    Inflammatory Response

o    Histology

·         Discussion

·         Acknowledgments

·         References