氢气治疗潜水减压病

Protective Effect of Hydrogen-Rich Saline on Decompression Sickness in Rats

氢气具有抗氧化应激的作用，而氧化应激在潜水减压病中发挥重要作用（几乎没有什么疾病与氧化没有有关）。本研究为验证氢气生理盐水是否具有预防潜水减压病的作用。研究方法：SD大鼠300-310克制备减压病模型。减压前24和12小时、减压时和减压后分别腹腔注射0.86mM氢气生理盐水10ml/kg（按照气体脱饱和理论，24和12小时的作用应该非常小）。结果发现，氢气盐水能显著降低减压病发病率（from 67.57 to 35.14%）。肺支气管肺泡灌洗液蛋白含量明显下降（from 0.33 +/- 0.05 to 0.14 +/- 0.01 mg /ml，MPO活性明显下降（from 0.86 +/- 0.16 to 0.44 +/- 0.13 U/g），肺组织MDA和8-OHdG明显下降。脊髓MDA明显下降。组织学改变显示氢气盐水能降低减压病组织损伤。结论：氢气盐水对潜水减压病可能具有治疗作用。

本研究具有重要意义：氢气本身就可用于潜水作业，既然氢气具有预防潜水减压病的作用，不仅对人们理解氢气潜水的意义，而且对氢气在其他类型潜水作业中都具有重要意义。例如在空气潜水前或减压过程或减压结束后，都可以适当给潜水员呼吸或饮用一定氢气或水。对大深度饱和潜水，更可以直接把氢气作为一种呼吸气体直接使用。

Introduction: Hydrogen (H-2) has been reported to be effective in the treatment of oxidative injury, which plays an important role in the process of decompression sickness (DCS). This study was designed to test whether H-2-rich saline (saline saturated with molecular hydrogen) protected rats against DCS. Methods: Models of DCS were induced in male Sprague-Dawley rats weighing 300-310 g. H-2-rich (0.86 mmol . L-1) saline was administered intraperitoneally (10 ml . kg(-1)) at 24 h, 12 h, immediately before compression, and right after fast decompression. Results: H-2-rich saline significantly decreased the incidence of DCS from 67.57 to 35.14% and partially counteracted the increases in the total concentration of protein in the bronchoalveolar lavage from 0.33 +/- 0.05 to 0.14 +/- 0.01 mg . ml(-1) (mean +/- SD; P < 0.05), myeloperoxidase activity from 0.86 +/- 0.16 to 0.44 +/- 0.13 U/g, levels of malondialdehyde (MDA) from 0.80 +/- 0.10 to 0.48 +/- 0.05 nmol . mg(-1), 8-hydroxydeoxyguanosine from 253.7 +/- 9.3 to 191.2 +/- 4.8 pg . mg(-1) in the lungs, and MDA level from 1.77 +/- 0.20 to 0.87 +/- 0.23 nmol . mg(-1) in the spinal cord in rat DCS models. The histopathology results also showed that H-2-rich saline ameliorated DCS injuries. Discussion: It is concluded that H-2-rich saline may have a protective effect against DCS, possibly due to its antioxidant action.