Hydrogen-rich saline ameliorates the severity of L-arginine-induced acute pancreatitis in rats

ABSTRACT

Background and Aims: Acute pancreatitis is an inflammatory process involving pancreatic tissue edema, acinar cell damage, neutrophil infiltration and the rapid production and release of inflammatory cytokines, which play a dominant role in local pancreatic inflammation and systemic complications. Molecular hydrogen, which reacts with the hydroxyl radical, a highly cytotoxic species produced in inflamed tissues, has been considered as a novel antioxidant. The study was to evaluate the therapeutic effects of hydrogen-rich saline on the L-arginine(Arg)-induced acute pancreatitis.

Methods: Acute pancreatitis was induced in Sprague-Dawley rats by giving two intraperitoneal injections of Arg, each at concentrations of 250 mg/100 g body weight, with a 1 h interval between injections. Rats were divided randomly into three groups: Normal, Arg plus saline treatment, and Arg plus hydrogen-rich saline treatment groups. Hydrogen-rich saline ( > 0.6 mM, 6 ml/kg) or saline (6 ml/kg) was administered respectively via tail vein in 15 minutes after each Arg administration. Pancreatitis severity was assessed by analysis of serum amylase, pancreatic water content, and histology. Samples of pancreas were also taken for measuring malondialdehyde and myeloperoxidase. Apoptosis in pancreatic acinar cell was determined with terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling technique (TUNEL). Expression and distribution of proliferating cell nuclear antigen (PCNA) and nuclear factor kappa B ( NF-κB) were detected with immunohistochemistry.

Results: Hydrogen-rich saline treatment significantly attenuated the severity of Arg-induced acute pancreatitis with inhibiting Arg-induced apoptosis, pancreatic tissue edema, activation of NF-κB and promoting acinar cells proliferation. Moreover, Hydrogen-rich saline treatment significantly limited the neutrophil infiltration, lipid oxidation and ameliorated the increased serum amylase.

Conclusions: These results indicate that hydrogen treatment has a protective effect against acute pancreatitis. This protective effect is possibly due to its ability to inhibit oxidative stress, apoptosis and to promote acinar cell proliferation.

Keywords: Hydrogen, Acute pancreatitis, Antioxidant, Oxidative stress, Apoptosis

 目前已经发表的氢气生物学效应的论著统计，红色为中国论文，兰色为日本，黑色为美国

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