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氢气对新生儿脑缺血没有作用？
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这个研究是美国的一个实验室与我们合作的论文。我一直关注这个研究。可惜发现的结果与我们的研究不一致。为什么这样，可能的原因是，呼吸的浓度高（2.9%），模型损伤比较重。明显比我们的损伤重。但这个解释似乎牵强。最近据说在手术损伤方面也没有发现治疗效果。真不可思议。值得庆幸的是，他们证明在大鼠缺血模型有效，但没日本报道的效果好。
Hydrogen gas is ineffective in moderate and severe neonatal hypoxia–ischemia rat models
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Gerald A. Matchetta, Nancy Fathalib, Yu Hasegawab, Vikram Jadhavb, Robert P. Ostrowskib, Robert D. Martina, Ihab R. Dorottaa, Xuejun Sund and John H. Zhanga, b, c,
, 
Accepted 12 December 2008.
Abstract
Hydrogen gas (H2) has been shown to ameliorate brain injury in experimental adult rat focal ischemia and in a mild neonatal hypoxia–ischemia (HI, 90 min hypoxia) rat model. In this study we tested H2 in moderate (120 min hypoxia) and severe (150 min hypoxia) neonatal HI rat models. We hypothesized that H2 would improve outcomes after neonatal HI by scavenging free radicals. Two hundred (200) unsexed Sprague–Dawley rats at day 10 of life (p10) underwent neonatal HI with the Rice–Vannucci model. Multiple treatment protocols were studied, including pre-ischemic treatment, intra-ischemic treatment, and post-ischemic treatment (Sham n = 32, HI n = 82, HI + H2 n = 86). We also tested H2 in middle cerebral artery occlusion (MCAO) in adult rats (MCAO n = 9, MCAO + H2 n = 7) for comparison. Analysis at 24 h included infarction volume, measurement of brain concentration of malondialdehyde (MDA) (an end-product of lipid peroxidation), daily weight, Nissl histology, and mortality. In moderate and severe neonatal HI models, hydrogen gas therapy (2.9% concentration H2) was not associated with decreased volume of infarction or decreased concentration of MDA. H2 gas pretreatment (2.9%) was associated with increased infarction volume in neonatal HI. In MCAO in adult rats, H2 gas therapy demonstrated a trend of beneficial effect. Exposure of H2 gas to non-ischemic neonates resulted in a significant increase in brain concentration of MDA. We conclude that 2.9% H2 gas therapy does not ameliorate moderate to severe ischemic damage in neonatal hypoxia–ischemia.
Keywords: H2; Hydrogen gas; Free radical; Neonatal hypoxia–ischemia
Abbreviations: HI, hypoxia–ischemia; MCAO, middle cerebral artery occlusion; MDA, malondialdehyde; PBS, Phosphate buffered saline; TTC, 2,3,5-triphenyltetrazolium chloride; RCAO, Right carotid artery occlusion
Article Outline
- 1. Introduction
- 2. Results
- 3. Discussion
- 4. Conclusion
- 5. Experimental procedures
- 5.1. Neonatal hypoxia–ischemia
- 5.2. Neonatal rat treatment methods
- 5.3. Adult rat middle cerebral artery occlusion
- 5.4. Adult rat treatment methods
- 5.5. Neurological score
- 5.6. Infarction volume
- 5.7. Measurement of lipid peroxidation
- 5.8. Histology
- 5.9. Statistical analysis
- Acknowledgements
- References
https://blog.sciencenet.cn/blog-41174-214205.html
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