氢分子医学分享 http://blog.sciencenet.cn/u/孙学军 对氢气生物学效应感兴趣者。可合作研究:sunxjk@hotmail.com 微信 hydrogen_thinker

博文

高压氧预适应保护脑缺血与抗氧化酶的关系

已有 6787 次阅读 2009-2-9 12:02 |个人分类:高压氧|系统分类:科研笔记| 论文, 高压氧, 预适应, 高压氧, 预适应

这个工作是与长海医院合作, 我们已经找到比较合理的暴露方案: 1小时/12小时连续4次. 我们这个方案是采用脊髓缺血模型找到的, 于是我们就采用经典的脑缺血模型(插线法),重复是否效果理想. 我们从各种抗氧化酶是否受高压氧诱导来解释这个效应,这个思路是来自四军大脊髓缺血模型的结果. 文章很快完成并发表在Brain res.

从线粒体途径的研究发表在神经科学上. 文章比较好一些.

Hyperbaric oxygen preconditioning induces tolerance against brain ischemia–reperfusion injury by upregulation of antioxidant enzymes in rats

Jiasi Lia, Wenwu Liub, Suju Dinga, Corresponding Author Contact Information, E-mail The Corresponding Author, Weigang Xub, Yangtai Guana, John H. Zhangc and Xuejun Sunb, Corresponding Author Contact Information, E-mail The Corresponding Author

aDepartment of Neurology, Changhai Hospital,174 Changhai Road, Shanghai 200433, PR China

bDepartment of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433, PR China

cDepartment of Neurosurgery, Loma Linda University, Loma Linda, California, USA


Accepted 4 March 2008. 
Available online 12 March 2008.


Abstract

The present study examined the hypothesis that cerebral ischemic tolerance induced by hyperbaric oxygen preconditioning (HBO-PC) is associated with an increase of antioxidant enzyme activity. Male Sprague–Dawley rats (250–280 g, n = 74) were divided into sham, middle cerebral artery occlusion (MCAO) for 90 min, and MCAO plus HBO-PC groups. HBO-PC was conducted four times by given 100% oxygen at 2.5 atmosphere absolute (ATA), for 1 h at every 12 h interval for 2 days. At 24 h after the last HBO-PC, MCAO was performed and at 24 h after MCAO, neurological function and Nissl Staining were performed to evaluate the effect of HBO-PC. Malondialdehyde (MDA) content, activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) sampled from the hippocampus, ischemic penumbra or core of cortex were measured. HBO-PC decreased mortality rate, improved neurological recovery, lessened neuronal injury, reduced the level of MDA and increased the antioxidant activity of CAT and SOD. These observations demonstrated that an upregulation of the antioxidant enzyme activity by HBO preconditioning plays an important role in the generation of tolerance against brain ischemia–reperfusion injury.

Keywords: Hyperbaric oxygen; Prevention; Antioxidant enzyme; Ischemic tolerance; Reactive oxygen species

Article Outline

1. Introduction
2. Results
2.1. Physiologic variables
2.2. Mortality rate and neurologic outcome
2.3. Nissl staining
2.4. MDA content
2.5. Antioxidant enzyme activities
3. Discussion
4. Experimental procedures
4.1. Animals and groups
4.2. HBO preconditioning
4.3. MCAO procedures
4.4. Neurologic functional scoring
4.5. Nissl staining
4.6. Ischemic core and penumbra dissections
4.7. Measurement of antioxidant enzyme activity and malondialdehyde content
4.8. Statistical analyses
Acknowledgements
Glossary
References
brain res

https://blog.sciencenet.cn/blog-41174-213785.html

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