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行家来说一下:南大超越韩春雨的基因编辑技术文章为什么发到Genome Biology?
韩春雨的基因编辑技术文章发到NBT(Nature Biotechnology)上,影响因子43.113。为什么超越韩春雨的基因编辑技术文章为什么发到Genome Biology上,其影响因子为11.313。
为什么不冲一下CNS呢?
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An alternative novel tool for DNA editing without...
An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease
Shu Xu†,Shasha Cao†,Bingjie Zou†,Yunyun Yue†,Chun Gu,Xin Chen,Pei Wang,Xiaohua Dong,Zheng Xiang,Kai Li,Minsheng Zhu,Qingshun Zhao and Guohua Zhou
Genome Biology201617:186;DOI: 10.1186/s13059-016-1038-5;Received: 23 May 2016,Accepted: 5 August 2016,Published: 15 September 2016
Engineered endonucleases are a powerful tool for editing DNA. However, sequence preferences may limit their application. We engineer a structure-guided endonuclease (SGN) composed of flap endonuclease-1 (FEN-1), which recognizes the 3′ flap structure, and the cleavage domain of Fok I (Fn1), which cleaves DNA strands. The SGN recognizes the target DNA on the basis of the 3′ flap structure formed between the target and the guide DNA (gDNA) and cut the target through its Fn1 dimerization. Our results show that the SGN, guided by a pair of gDNAs, cleaves transgenic reporter gene and endogenous genes in zebrafish embryonic genome.
DNA-guided genome editing using structure-guided...
DNA-guided genome editing using structure-guided endonucleases
Gaurav K. Varshney and Shawn M. Burgessr
Genome Biology201617:187;DOI: 10.1186/s13059-016-1055-4;Published: 15 September 2016
The search for novel ways to target and alter the genomes of living organisms accelerated rapidly this decade with the discovery of CRISPR/Cas9. Since the initial discovery, efforts to find alternative methods for altering the genome have expanded. A new study presenting an alternative approach has been demonstrated that utilizes flap endonuclease 1 (FEN-1) fused to the Fok1 endonuclease, which shows potential for DNA-guided genome targeting in vivo.
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