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Genome-wide association study dissecting drought resistance-associated loci based on physiological traits in common bean 基于生理性状挖掘普通菜豆抗旱相关QTL |
Lei Wu1, 2, Yujie Chang2, Lanfen Wang2, Shumin Wang2, Jing Wu2, 3# |
1 Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China 3 SXAU–ICS (Shanxi Agricultural University–Institute of Crop Sciences) Joint Research Center of Minor Crops, Shanxi Agricultural University, Taiyuan 030031, China |
摘要
抗旱性遗传改良是普通菜豆育种的主要目标之一,因此,亟需挖掘与抗旱性相关的分子标记,通过分子辅助育种技术促进普通菜豆抗旱育种。本研究测定了210份普通菜豆的脯氨酸、海藻糖、棉子糖和水苏糖含量,发现不同处理条件下的脯氨酸、海藻糖、棉子糖和水苏糖含量变化较大。对照条件下脯氨酸含量的变异系数最小(21.21%),水苏糖含量的变异系数最大(78.69%),干旱条件下海藻糖含量的变异系数最大(50.08%),脯氨酸含量的变异系数最小(20.11%)。通过全基因组关联分析鉴定出32个数量性状位点与抗旱性相关,其中7个与已知抗旱相关遗传位点重叠。在染色体Pv01、Pv07和Pv11上发现了4个热点区域。结合基因功能注释、同源基因功能研究以及基因表达谱数据,在候选区段中鉴定出多个候选基因,包括编码MYB、bZIP、bHLH、ERF和蛋白激酶的基因。其中Phvul.001G189400、Phvul.007G273000和Phvul.008G270500分别被注释为bZIP、ERF和WRKY,这些基因参与拟南芥的干旱胁迫反应,在普通菜豆中受干旱胁迫诱导。基于候选基因区域的显著SNP,可以将供试材料分成不同的单倍型,表型分析结果表明不同单倍型之间存在显著差异。这些结果丰富了普通菜豆抗旱性遗传信息,鉴定出来的候选基因和优良的自然变异将有助于普通菜豆抗旱性改良。
Abstract
Genetic improvement of drought resistance is one of the main breeding goals for common bean, so molecular markers must be identified to facilitate drought resistance breeding. In this study, we evaluated the proline, trehalose, raffinose, and stachyose contents of 210 common bean accessions under two watering conditions and found large variations in all four. The coefficients of variation ranged from 21.21% for proline content to 78.69% for stachyose content under well-watered conditions, and from 20.11% for proline content to 50.08% for trehalose content under drought stress. According to our genome-wide association analysis, 32 quantitative trait loci were associated with drought resistance, seven of which overlapped with known loci. Four hotspot regions were identified at Pv01, Pv07 and Pv11. A set of candidate genes was identified, including genes encoding MYB, bZIP, bHLH, ERF, and protein kinases. Among these genes, Phvul.001G189400, Phvul.007G273000 and Phvul.008G270500 were annotated as bZIP, ERF and WRKY, respectively. These genes are reportedly involved in drought stress responses in Arabidopsis thaliana and were induced by drought stress in common bean. Significant SNPs in six candidate gene regions formed different haplotypes, and phenotypic analysis revealed significant differences among the haplotypes. These results provide new insight into the genetic basis of drought resistance in common bean and reveal candidate genes and superior natural variations that will be useful for improving common bean.
全文链接:https://www.sciencedirect.com/science/article/pii/S2095311924001515
Journal of Integrative Agriculture (《农业科学学报》(英文), JIA) 由中华人民共和国农业农村部主管,中国农业科学院与中国农学会主办,中国农业科学院农业信息研究所承办。综合性英文学术期刊,月刊。创刊于2002年,现任主编为中国科学院院士陈化兰。JIA主要栏目有作物科学、园艺、植物保护、动物科学、动物医学、农业生态环境、食品科学、农业经济与管理等。刊稿类型有综述、研究论文、简报以及评述等。全部论文在Elsevier-ScienceDirect (SD) 平台OA出版。最新SCI影响因子4.6,位于SCI-JCR农业综合学科Q1区。中国科学院分区农林科学1区。2016年以来先后获得中国科协等部委 “提升计划”“登峰计划”“卓越计划”项目支持。
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