2009年4月的IJSEM期刊上发表了Vidal等人的研究论文，报道了中慢生根瘤菌属的一个新种，即耐重金属中慢生根瘤菌(Mesorhizobium metallidurans)。该种与天山中慢生根瘤菌(Mesorhizobium tianshanense)的系统发育关系接近，见下图。该新种对重金属的抗性是：Tolerates high concentrations of heavy metals: 16–32 mM zinc and 0.3–0.5 mM cadmium。分离自重金属污染的土壤（heavy metal-enriched spoil heaps at the Saint Laurent le Minier mine (Languedoc, France). ）

Int J Syst Evol Microbiol 59 (2009), 850-855; DOI  10.1099/ijs.0.003327-0
© 2009 International Union of Microbiological Societies

Mesorhizobium metallidurans sp. nov., a metal-resistant symbiont of Anthyllis vulneraria growing on metallicolous soil in Languedoc, France

Céline Vidal¹, Clémence Chantreuil², Odile Berge³, Lucette Mauré¹, José Escarré⁴, Gilles Béna², Brigitte Brunel⁵ and Jean-Claude Cleyet-Marel¹

¹ INRA, USC1242, Laboratoire des Symbioses Tropicales et Méditerranéennes, Campus International de Baillarguet TA A-82/J, F-34398, Montpellier Cedex 5, France
² IRD, UMR 113, Laboratoire des Symbioses Tropicales et Méditerranéennes, F-34398 Montpellier, France
³ CNRS, UMR 6191, Laboratoire d'Ecologie Microbienne de la Rhizosphère et des Environnements Extrêmes, F-13108 Saint-Paul lez Durance, France
⁴ CNRS, UMR 5175, Centre d'Ecologie Fonctionnelle et Evolutive, 1919 Route de Mende, F-34293 Montpellier, France
⁵ Montpellier SupAgro, UMR 113, Laboratoire des Symbioses Tropicales et Méditerranéennes, F-34398 Montpellier, France

 

Correspondence
Jean-Claude Cleyet-Marel
cleyet@supagro.inra.fr

 

A polyphasic taxonomic approach was used to characterize 31 rhizobial isolates obtained from Anthyllis vulneraria, a metallicolous legume species, growing close to a zinc mine in the south of France (Saint Laurent le Minier). Comparative analysis of nearly full-length 16S rRNA gene sequences showed that these Gram-negative bacteria belonged to the genus Mesorhizobium and that they were related most closely to Mesorhizobium tianshanense ORS 2640^T. The phylogenetic relationships of these isolates with other Mesorhizobium species were confirmed by sequencing and analysis of the recA and atpD genes, which were used as alternative chromosomal markers. These novel mesorhizobial strains tolerated high concentrations of heavy metals: 16–32 mM Zn and 0.3–0.5 mM Cd. DNA–DNA hybridizations revealed >73 % relatedness between the strains isolated from A. vulneraria, but only 19–33 % relatedness between these and the type strains of M. tianshanense and Mesorhizobium mediterraneum. These results, together with other phenotypic characteristics, support the conclusion that these isolates represent a single, novel species of the genus Mesorhizobium, for which the name Mesorhizobium metallidurans sp. nov. is proposed. The type strain is STM 2683^T (=CFBP 7147^T=LMG 24485^T).

该种的系统发育地位见图：

 

Anthyllis vulneraria这种植物的图片见下（来源于http://www.plant-identification.co.uk/skye/leguminosae/anthyllis-vulneraria.htm）：

 

Range	Most of Europe, including Britain, south and east to the Caucasus and N. Africa.
Habitat	Dry situations on sea cliffs, stony rocks and shingle, usually on shallow calcareous soils

It is hardy to zone 7 and is not frost tender. It is in flower from June to September, and the seeds ripen from July to September. The flowers are hermaphrodite (have both male and female organs) and are pollinated by Bees, Lepidoptera (Moths & Butterflies). It can fix Nitrogen. It is noted for attracting wildlife.

The plant prefers light (sandy) and medium (loamy) soils, requires well-drained soil and can grow in nutritionally poor soil. The plant prefers neutral and basic (alkaline) soils and can grow in very alkaline soil. It cannot grow in the shade. It requires dry or moist soil and can tolerate drought. The plant can tolerate maritime exposure.

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