Trypsin | 胰蛋白酶

MCE 国际站：Trypsin

中文名：胰蛋白酶

CAS：9002-07-7

品牌：MedChemExpress (MCE)

存储条件：Please store the product under the recommended conditions in the Certificate of Analysis.

生物活性：胰蛋白酶是一种水解赖氨酸或精氨酸羧基侧蛋白质的酶。胰蛋白酶激活 PAR2 和 PAR4。胰蛋白酶通过 PDCoV 的 S 糖蛋白与 pAPN 的相互作用在 PDCoV 感染中诱导细胞间膜融合。胰蛋白酶还促进细胞增殖和分化。胰蛋白酶可用于伤口愈合和神经源性炎症的研究[1][2][3][4][6]。 IC50 和靶标：PAR2、PAR4[6]  

体外：胰蛋白酶（5 μg/mL，24 或 48 小时）促进猪三角型冠状病毒 (PDCoV) 在 LLC-PK 细胞中的复制^[2]。胰蛋白酶（10 和 50 ng/mL，12 小时) 通过促进 LLC-PK 细胞的膜融合来增强 PDCoV 细胞间的传播^[2]。胰蛋白酶 (0.05%, 3 h) 促进 C6 神经胶质瘤细胞在无血清和无生长因子培养基中增殖^[3]。胰蛋白酶（20 -150 ng/mL，5 天）增强 PBMC 分化^[4].

体内：胰蛋白酶（在 50 μL 盐水中每个位点 100-500 μg，皮内注射）诱导小鼠的抓挠行为^[5]。

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研究领域：Metabolic Enzyme/Protease  |  GPCR/G Protein

作用靶点：Ser/Thr Protease  |  Protease Activated Receptor (PAR)

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参考文献：[1]. Bhupendra S.Kaphalia. Chapter 16 - Biomarkers of acute and chronic pancreatitis. Biomarkers in Toxicology. 2014, Pages 279-289.

 [2]. Yue-Lin Yang,et al. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerg Microbes Infect. 2020 Feb 24;9(1):457-468.

 [3]. H Amano, et al. Trypsin promotes C6 glioma cell proliferation in serum- and growth factor-free medium. Neurosci Res. 1996 Jul;25(3):203-8. 

[4]. Michael J. V. White, et al. Trypsin Potentiates Human Fibrocyte Differentiation. PLoS One. 2013; 8(8): e70795. 

[5]. R Costa, et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice. Br J Pharmacol. 2008 Jul;154(5):1094-103. 

[6]. F Schmidlin, et al. Protease-activated receptors: how proteases signal to cells. Curr Opin Pharmacol. 2001 Dec;1(6):575-82. 

[7]. Bhupendra S.Kaphalia, et al. Chapter 16 - Biomarkers of acute and chronic pancreatitis. Biomarkers in Toxicology. 2014, Pages 279-289.