Comparison of Three Strategies for Sequencing Rabies Viral G Gene（狂犬病毒G基因序列测定三种策略的比较）

Shengli Meng, Wenli Lv, Jie Wu, Jilin Wang, Gelin Xu, Jiaxin Yan

（Wuhan Institute of Biological Products Co., Ltd.,Wuhan , P.R . China，430207 China）

Journal of Applied Virology 2（1）：15－24 (2013)

Abstract

It is essential to rapidly, low-cost and precisely determining gene sequences of rabies virus. Reverse transcription-polymerase chain reaction can be used to identify rabies viral G gene sequences. In this study, we evaluated three methods, conventional RT-PCR and direct sequencing, adapter RT-PCR and sequencing with universal primers, conventional RT-PCR and cloning sequencing with universal primers, to detect rabies in animal brain homogenate. Four rabies isolates recovered from Fuyang city of Anhui province were diagnosed as positive using the fluorescent antibody test, rapid rabies enzymeimmunodiagnosis methods and the mouse inoculation test. The results indicated that the adapter RT-PCR method and sequencing with universal primers is extremely well suited for sequencing rabies viral gene, which is rapid,cost-effective and precise compared with other two methods.

Journal ofApplied Virology (Print ISSN: 2305-5154, Online ISSN 2306-6210)

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