番茄PLC基因家族鉴定及抗番茄褐色皱果病毒（ToBRFV）防御反应分析

• 方远鹏  ,  

• 韦建明  ,  

• 李云洲 

作者信息 +

Identification of PLC Gene Family and Analysis of Defense Response Against Tomato Brown Rugose Fruit Virus (ToBRFV) in Tomato

• FANG Yuanpeng  ,  

• WEI Jianming  ,  

• LI Yunzhou 

Author information +

文章历史 +

番茄褐色皱果病毒（ToBRFV）被我国列为检疫性病毒，严重威胁番茄的安全生产。为了明确番茄磷脂酶C（PLC）的种类，探究番茄PCL在植株抗ToBRFV防御反应过程中的潜在作用。本研究首先基于生物信息学鉴定了10个番茄PLC家族成员，其中特异性磷脂酶C（PI-PLC）7个，非特异性磷脂酶C（NPC）3个，7个PI-PLC蛋白均具备3个核心结构域（PLC_X c、PLC_Y c、C2）和1个EF_hand-like结构域，3个NPC蛋白均只具有Phosphoesterase结构域。10个番茄PLC蛋白按照结构相似度可以划分为7个分支，分别为NPC1、NPC2、NPC6、PI-PLC2、PI-PLC3、PI-PLC4、PI-PLC6。另外，10个番茄PLC蛋白的二级结构占比类似，但三级结构存在明显差异。共线性分析结果显示，番茄PLC基因与水稻、拟南芥、雷蒙德式棉PLC基因间分别存在3、12、16对共线性关系。最后，通过转录组测序方法，检测了PLC基因家族在接种ToBRFV后的相对表达水平，结果显示，SlNPC1、SlNPC6、SlPLC4在ToBRFV接种的样本中表达水平较高，其他PLC基因则在ToBRFV接种后表达水平降低。本研究为番茄抗ToBRFV研究和育种奠定了技术与理论基础。

Tomato brown rugose fruit virus （ToBRFV） belongs to the genus Tobamovirus in the Virgaviridae family and is classified as a quarantine virus in China， posing a serious threat to the safety of tomato production. Signal transduction system plays an important role in plant disease resistance， and phospholipid signaling is crucial in the process of extracellular resistance signal transduction. Phospholipase C （PLC） is critical in the transmembrane signaling of the phospholipid signaling system. In this study， we first identified 10 tomato PLC genes based on bioinformatics， including seven phosphati-dylinositol-specific phospholipase C （PI-PLC） and three non-specific phospholipase C （Non-specifc -PLC， NPC）， the seven PI-PLC PLC proteins have three core structural domains （PLC_X c， PLC_Y c， C2） and an EF_hand-like structural domain， and the three NPC proteins only have a phosphoesterase structural domain. 10 tomato PLC proteins can be divided into seven branches according to their structural similarity， namely NPC1， NPC2， NPC6， PI-PLC2， PI-PLC3， PI-PLC4 and PI-PLC6. In addition， the 10 tomato PLC secondary structures were similar in proportion， but there were significant differences in tertiary structures. Co-linearity analysis showed that there were 3， 12 and 16 pairs of co-linear relationships between the distribution of tomato PLC genes and rice， Arabidopsis and Raymond-type cotton PLC genes. Finally， to clarify which tomato PLCs are involved in the plant defense response against ToBRFV， this study examined the relative expression levels of the PLC gene family after ToBRFV inoculation by transcriptome sequencing， and the results showed that SlNPC1， SlNPC6 and SlPLC4 were expressed at higher levels in the ToBRFV-inoculated samples， while the expression levels of other PLC genes were reduced after ToBRFV inoculation. This study lays the technical and theoretical foundation for tomato ToBRFV resistance research and breeding.

番茄 / PLC基因家族 / 生物信息学 / 抗病毒 / ToBRFV

tomato / PLC gene family / bioinformatics / anti-virus / ToBRFV

导出引用

方远鹏 , 韦建明 , 李云洲. 番茄PLC基因家族鉴定及抗番茄褐色皱果病毒（ToBRFV）防御反应分析[J]. 核农学报, 2023, 37(2): 230-240 https://doi.org/10.11869/j.issn.1000-8551.2023.02.0230

FANG Yuanpeng , WEI Jianming , LI Yunzhou. Identification of PLC Gene Family and Analysis of Defense Response Against Tomato Brown Rugose Fruit Virus (ToBRFV) in Tomato[J]. Journal of Nuclear Agricultural Sciences, 2023, 37(2): 230-240 https://doi.org/10.11869/j.issn.1000-8551.2023.02.0230