狼蛛肌肉中的酶活性模式

Enzymes of the glycolytic pathway, of the citric acid cycle, and of related metabolic reactions have been measured in muscles of the femur and prosoma, and in the heart of Cupiennius. The data are summarized in Fig. 1 and in Table 1. The energy yielding metabolism of the muscles may be characterized as solely glycolytic, with high capacity for lactic acid production. Hexokinase activity is low in the femur and prosoma, but somewhat higher in the heart. Mitochondrial enzyme activities are extremely low. The lack of mitochondria is confirmed by electron micrographs. Since malate dehydrogcnase occurs with very high activity, Sacktor's (1965) scheme for regeneration of NAD + is considered. Of the transaminases required, GOT has high, GPT medium activity. The anabolic capacity of the spider heart (haemocyte development) is reflected by its higher activities of NADPH generating enzymes. The biological significance of these findings is discussed. Wilson and Bullock's (1973) hypothesis on the causes of locomotory collapse in spiders is rejected in the case of Cupiennius, since it is the biochemical construction of the leg muscles rather than circulatory failure which causes rapid exhaustion.

糖酵解途径的酶，柠檬酸循环的酶，以及相关的代谢反应的酶，已经在(蜘蛛)股骨和前体的肌肉中以及在狼蛛Cupiennius的心脏中被测量到了。数据汇总在图1和表1中。肌肉的能量代谢可以被描述为单纯的糖酵解，具有很高的乳酸产生能力。己糖激酶活性在蜘蛛股骨和前体中较低，但在心脏中略高。线粒体酶活性极低。电子显微镜证实线粒体的缺失。由于苹果酸脱氢酶具有很高的活性，因此Sacktor(1965)提出的NAD+再生方案是可以考虑的。在所需的转氨酶中，谷草转氨酶(GOT)活性较高，谷丙转氨酶GPT中等活性。蜘蛛心脏的合成代谢能力(血细胞发育)反映在其较高的NADPH生成酶活性上。讨论了这些发现的生物学意义。威尔逊和布洛克(1973)关于蜘蛛运动崩溃原因的假说在Cupiennius的例子中被拒绝了，因为导致快速精疲力竭的是腿部肌肉的生化结构，而不是循环衰竭。

Linzen, B., Gallowitz, P., 1975. Enzyme activity patterns in muscles of the lycosid spider,Cupiennius salei. Journal of comparative physiology 96, 101-109.