饲料脂质水平对塞内加尔比目鱼生长相关基因的表达有显著影响

In Senegalese sole (Solea senegalensis Kaup), growth is negatively correlated to dietary lipid levels. To understand the molecular basis of this effect a molecular toolbox of 12 genes, including fgf6, fst, mstn1, myf5, mrf4, myod1, myod2, myog, myHC, mylc2, igf1r and insr, was developed. The expression profiles of these genes were investigated in white muscle and liver of fish fed with three dietary lipid levels (4%, 12% and 20%). The expression of igf-I and igf-II was also examined. MRFs and myosins were only expressed in the muscle and, except for myf5, the general trend was a decrease in expression with an increase in dietary lipids. Fgf6 was identified for the first time in liver and its expression augmented in hepatic tissues with increasing dietary lipid levels. A similar tendency was observed for mstn1 and igf-I. The opposite was observed for igf1r expression in muscle and liver. Myog, mrf4, mylc2 and igf1r were highly correlated with growth and nutrient utilisation indices. In addition to its practical implications, this work provides a valuable contribution towards our understanding of the genetic networks controlling growth in teleosts.

在塞内加尔比目鱼(Solea Senegalensis Kaup)中，生长与饲料脂肪水平呈负相关。为了了解这种效应的分子基础，我们开发了一个包含12个基因的分子工具箱，包括fgf6、fst、mstn1、myf5、mrf4、myod1、myod2、myog、myHC、myc2、IGF1r和insr。研究了3种脂肪水平(4%、12%和20%)饲料喂食的的鱼体内白肌和肝脏中这些基因的表达谱。同时检测IGF-I和IGF-II的表达。MRFs和肌球蛋白只在肌肉中表达，除myf5外，总的趋势是随着膳食脂质的增加而表达减少。fgf6在肝脏中首次被发现，其在肝组织中的表达随日粮脂质水平的升高而增强。Mstn1和IGF-I也有类似的趋势。IGF1R在肌肉和肝脏中的表达则相反。MYOG、MRF4、MYLC2和IGF1R与生长和养分利用指数高度相关。除了它的实际意义之外，这项工作还为我们理解控制硬骨鱼生长的遗传网络提供了有价值的贡献。

Campos, C., Valente, L.M., Borges, P., Bizuayehu, T., Fernandes, J.M., 2010. Dietary lipid levels have a remarkable impact on the expression of growth-related genes in Senegalese sole (Solea senegalensis Kaup). The Journal of experimental biology 213, 200-209.