《International Immunopharmacology》：岩藻多糖可以保护阿司匹林引发的胃黏膜损伤

原标题：岩藻多糖对阿司匹林引起的胃黏膜损伤的免疫保护作用

①  非甾类抗炎药物（如阿司匹林）的大量使用也是导致胃溃疡及相关疾病的重要原因，可引发胃溃疡、胃出血等症状；

②  岩藻多糖是来自于褐藻中的含硫酸基的多糖物质，包括岩藻糖、半乳糖等，具有广泛的生物活性，包括保护胃黏膜、清除幽门螺杆菌等作用；

③  本文研究了岩藻多糖对阿司匹林诱发的胃黏膜损伤的免疫保护作用，先给小鼠每天喂食岩藻多糖，持续14天，之后灌胃阿司匹林，观察小鼠胃黏膜损伤情况；

④  实验结果显示，小鼠喂食阿司匹林后，小鼠体内氮氧化合物水平、白细胞介素-4、6、10、12水平、肿瘤坏死因子-α水平、干扰素-γ水平都发生显著急性变化，而这些症状在提前服用岩藻多糖的小鼠体内得到有效抑制，从而说明岩藻多糖对阿司匹林诱发的胃黏膜损伤具有很好地保护作用。

关键词：岩藻多糖；胃黏膜；阿司匹林；非甾类抗炎药

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International Immunopharmacology 11 (2011) 157–163.

Immunomodulatory activity of fucoidan against aspirin-induced gastric mucosal damage in rats

Abstract:

Gastric ulcers and related complications associated with the use of non-steroidal anti-inflammatory drugs (NSAIDs), such as aspirin, represent a major global health problem. In the present study, we investigate the immunological activity of fucoidan against aspirin-induced gastric mucosal damage in rats. Thirty-six rats were randomly divided into the following, normal (Carboxy methyl cellulose 0.05 %), aspirin (Asp—400 mg/kg) treated, fucoidan alone (Fu—0.02 g/kg, daily for 14 days) and Fu+Asp. Cytokines, total nitrite and nitrate (NOx) analysis and tissue localization of Cyclooxygenase 1, 2 and epidermal growth factor receptor (EGFR) were done using Elisa and immunohistochemistry respectively. Histopathology of gastric tissue, collagen deposition was performed using Hematoxylin and Eosin andMasson's trichromewere performed. Treatment of ratswith a single dose of aspirin (400mg/kg, orally) led to significant alterations in the levels of total nitrite and nitrate (NOx), interleukins (IL-4, 6, 10, 12), tumor necrosis factor (TNF-α), and interferon gamma (IFN-γ). Notably, collagen deposition in glandular tissue and localization of cyclooxygenase 1, 2, and epidermal growth factor were considerably affected in aspirin-treated rats. These severities were prevented to a significant extent in rats pretreatedwith fucoidan (0.02 g/kg/day for twoweeks orally). Our findings collectively indicate that the gastroprotective effect of fucoidan against aspirin-induced ulceration in rats is mediated through its immunomodulatory properties.

All Authors:

Hanumantha Rao Balaji Raghavendran, Periasamy Srinivasan, Sathyanath Rekha