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the plant journal:玉米ZmLBD5基因通过抑制ABA合成负调控干旱耐受性

已有 1972 次阅读 2022-11-1 22:48 |个人分类:每日摘要|系统分类:论文交流

ZmLBD5, a class-II LBD gene, negatively regulates drought tolerance by impairing abscisic acid synthesis

第一作者Xuanjun Feng

第一单位西南作物基因资源发掘与利用国重实验室

通讯作者Yanli Lu


 Abstract 

背景回顾:Lateral organ boundaries domain (LBD) proteins are plant-specific transcription factors. Class-I LBD members have been widely demonstrated to play pivotal roles in organ development; however, knowledge on class-II members remains limited. 


主要发现Here, we report that ZmLBD5, a class-II LBD member, is involved in the regulation of maize growth and drought response by affecting gibberellin (GA) and abscisic acid (ABA) synthesis. 


结果1-ZmLBD5作用机制ZmLBD5 is mainly involved in regulation of the TPS-KS-GA2ox gene module, which comprises key enzymatic genes upstream of GA and ABA biosynthesis. 


结果2-ZmLBD5过表达和敲除表型The insufficiency of ABA increases stomatal density and aperture in overexpressed plants, and causes the drought sensitive phenotype by promoting water transpiration. The increase of GA1 promoted seedling growth in overexpressed plants. Accordingly, the CRISPR/Cas9 knockout lbd5 seedlings were dwarf but drought tolerant. 


结果3-籽粒产量Moreover, lbd5 has larger grain yield under drought stress and no penalty in well watered conditions than the wild type.


结论On the whole, ZmLBD5 is a negative regulator in maize drought tolerance, and it is a potentially useful target for drought resistance breeding.


 摘 要 

侧生器官边界结构域(LBD)蛋白是植物特异性转录因子。I类LBD基因被广泛证明在器官发育中发挥重要作用,但是有关II类LBD基因的作用还不清楚。本文中,作者发现玉米中一个II类LBD基因ZmLBD5通过影响赤霉素(GA)和脱落酸(ABA)合成来参与玉米的生长和干旱响应调控。ZmLBD5主要参与TPS-KS-GA2ox基因模块的调控,而后者是GA和ABA生物合成上游的关键酶编码基因。ZmLBD5过表达植株中ABA的不足导致气孔密度和开度的增强,从而通过促进水分蒸腾而增强了干旱敏感性。另外,ZmLBD5过表达植株中GA1水平的增加促进幼苗的生长。与此同时,利用CRISPR/Cas9敲除ZmLBD5基因获得的lbd5突变体存在矮化表型,但是其干旱耐受性增强。此外,与野生型相比,lbd5突变体在干旱条件下的籽粒产量更高,而且在水分充足条件下与野生型产量相当。综上,ZmLBD5基因是玉米干旱耐受性的负调控因子,并且其是玉米干旱抗性育种的潜在靶标。




** 卢艳丽 **


个人简介:

2001-2005年,四川农业大学,学士;

2005-2010年,四川农业大学,博士;

2010-2012年,四川农业大学玉米研究所,副研究员;

2012年-至今,四川农业大学玉米研究所,研究员。


研究方向:主要从事玉米重要农艺性状和抗逆性状的遗传解析与分子调控网络研究,基因克隆与转基因功能验证以及分子育种等研究工作。


doi: https://doi.org/10.1111/tpj.16015


Journal: the plant journal 

Published date: October 28, 2022


Cite:
Xuanjun Feng, Jing Xiong, Weixiao Zhang, Huarui Guan, Dan zheng, Hao Xiong, Li Jia, Yue Hu, Hanmei Zhou, Ying Wen, Xuemei Zhang, Fengkai Wu, Qingjun Wang, Jie Xu, Yanli Lu. ZmLBD5, a class-II LBD gene, negatively regulates drought tolerance by impairing abscisic acid synthesis. the plant journal, 2022. DOI: https://doi.org/10.1111/tpj.16015



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