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The Plant Cell:磷酸酶PP2C D6和D7直接与SOS1互作并抑制其活性

已有 975 次阅读 2022-9-26 23:14 |个人分类:每日摘要|系统分类:论文交流

SALT OVERLY SENSITIVE 1 is inhibited by clade D Protein phosphatase 2C D6 and D7 in Arabidopsis thaliana

第一作者Haiqi Fu

第一单位中国农业大学

通讯作者Yan Guo


 Abstract 

背景回顾:The Salt Overly Sensitive (SOS) pathway is essential for maintaining sodium ion homeostasis in plants. This conserved pathway is activated by a calcium signaling-dependent phosphorylation cascade. 


提出问题However, the identity of the phosphatases and their regulatory mechanisms that would deactivate the SOS pathway remain unclear. 


结果2-PP2C D6/D7In this study, we demonstrate that PP2C.D6 and PP2C.D7, which belong to clade D of the protein phosphatase 2C (PP2C) subfamily in Arabidopsis thaliana, directly interact with SOS1 and inhibit its Na+/H+ antiporter activity under non-salt-stress conditions. 


结果2-ScaBP8Upon salt stress, SOS3-LIKE CALCIUM BINDING PROTEIN8 (SCaBP8), a member of the SOS pathway, interacts with the PP2Cs and suppresses their phosphatase activity; simultaneously, SCaBP8 regulates the subcellular localization of PP2C.D6 by releasing it from the plasma membrane. 


结论1Thus, we identified two negative regulators of the SOS pathway that repress SOS1 activity under non-stress conditions. These processes set the stage for the activation of SOS1 by the kinase SOS2 to achieve plant salt tolerance. 


结论2Our results suggest that reversible phosphorylation/dephosphorylation is crucial for the regulation of the SOS pathway, and that calcium sensors play dual roles in activating/deactivating SOS2 and PP2C phosphatases under salt stress.


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 摘 要 

盐过敏SOS途径对于维持植物钠离子稳态至关重要。该保守途径受到钙离子信号转导依赖性磷酸化级联所激活。但是,作用于SOS途径去磷酸化的磷酸酶及其调控机制还不清楚。本文中,作者发现在非盐胁迫条件下,拟南芥蛋白磷酸酶PP2C家族的D分枝蛋白PP2C.D6和PP2C.D7能够直接与SOS1互作,并抑制SOS1的钠离子/氢离子逆向转运活性。在盐胁迫下,SOS途径中的SOS3类似钙结合蛋白SCaBP8能够与PP2Cs互作,抑制PP2Cs蛋白的磷酸酶活性;同时,SCaBP8通过将PP2C.D6从质膜上释放来调控PP2C.D6的亚细胞定位。因此,作者鉴定到了SOS途径的两个负调控因子,可在非盐胁迫条件下抑制SOS1的活性。这些过程为激酶SOS2激活SOS1以实现植物耐盐性创造了条件。本文的研究结果揭示了磷酸化/去磷酸化的可逆性对于SOS途径的调控至关重要,且钙离子感受器在盐胁迫下激活/失活SOS2和PP2C磷酸酶中发挥双重作用。




** 郭 岩 **


个人简介:

1988年,北京农业大学,学士;

1988-1995年,中科院遗传所,研究助理;

1995-1999年,德国科隆大学、马普植物育种所,博士;

1999-2003年 ,美国亚利桑那大学,博后;

2004-2009年,北京生命科学研究所,研究员;

2009年-至今,中国农业大学,教授。


研究方向:植物如何感受并响应环境胁迫,诸如土壤高浓度盐碱和干旱,特别是植物在胁迫条件下体内钙离子信号和pH内平衡的调节。


doi: https://doi.org/10.1093/plcell/koac283


Journal: The Plant Cell

Published date: September 23, 2022


Cite:
Haiqi Fu, Xiang Yu, Yuanyuan Jiang, Yuhan Wang, Yongqing Yang, She Chen, Qijun Chen, Yan Guo. SALT OVERLY SENSITIVE 1 is inhibited by clade D Protein phosphatase 2C D6 and D7 in Arabidopsis thaliana. The Plant Cell, 2022. DOI: https://doi.org/10.1093/plcell/koac283



https://blog.sciencenet.cn/blog-3158122-1356960.html

上一篇:Current Opinion in Plant Biology:植物适应环境的隐藏时间动态
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