背景回顾：One of the hallmarks of plant senescence is the global transcriptional reprogramming coordinated by a plethora of transcription factors (TFs). 

提出问题：However, mechanisms underlying the interactions between different TFs in modulating senescence remain obscure. 

前期基础：Previously, we discovered that plant ABS3 subfamily MATE transporter genes regulate senescence and senescence-associated transcriptional changes. 

结果1-突变体库筛选：In a genetic screen for mutants suppressing the accelerated senescence phenotype of the gain-of-function mutant abs3-1D, AUXIN RESPONSE FACTOR 2 (ARF2) and PHYTOCHROME-INTERACTING FACTOR 5 (PIF5) were identified as key TFs responsible for transcriptional regulation in the ABS3-mediated senescence pathway. 

结果2-ARF2-PIF5-ABS3：ARF2 and PIF5 (as well as PIF4) interact directly and function interdependently to promote senescence, and they share common target genes such as key senescence promoting genes ORESARA 1 (ORE1) and STAY-GREEN 1 (SGR1) in the ABS3-mediated senescence pathway. In addition, we discovered reciprocal regulation between ABS3-subfamily MATEs and the ARF2 and PIF5/4 TFs. 

结论：Taken together, our findings reveal a regulatory paradigm in which the ARF2-PIF5/4 functional module facilitates the transcriptional reprogramming in the ABS3-mediated senescence pathway.

 摘 要 

植物衰老的标志之一是由大量转录因子协调的全局转录重编程。然而，不同TF之间相互作用以调控植物衰老的机制仍然不清楚。此前，作者发现植物ABS3亚家族MATE转运蛋白基因调节衰老和衰老相关的转录变化。在突变体筛库过程中，作者获得了一个能够抑制abs3-1D功能获得突变体的加速衰老表型的突变体，经鉴定生长素响应因子ARF2和光敏色素相互作用因子PIF5是ABS3介导的植物衰老途径中涉及转录调控的关键TF。ARF2和PIF5（以及PIF4）之间能够发生直接互作，并且相互依赖地促进植物衰老，它们之间享有共同的靶基因，如ABS3介导的植物衰老途径中的关键衰老促进基因ORE1和SGR1。此外，作者还发现ABS3亚家族MATEs和ARF2及PIF5/4等转录因子之间存在相互调节。综上所述，本文的研究结果揭示了一种分子调控途径，其中ARF2-PIF5/4功能模块促进ABS3介导的植物衰老途径中的转录重编程。

 郁 飞