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PNAS:谷类作物中,EGT1基因通过反重力性机制控制根角度

已有 955 次阅读 2022-8-2 09:20 |个人分类:每日摘要|系统分类:论文交流

Root angle is controlled by EGT1 in cereal crops employing an antigravitropic mechanism

第一作者Riccardo Fusi

第一单位诺丁汉大学

通讯作者Malcolm J. Bennett


 Abstract 

背景回顾Root angle in crops represents a key trait for efficient capture of soil resources. Root angle is determined by competing gravitropic versus antigravitropic offset (AGO) mechanisms. 


主要发现Here we report a root angle regulatory gene termed ENHANCED GRAVITROPISM1 (EGT1) that encodes a putative AGO component, whose loss-of-function enhances root gravitropism. 


结果1-EGT1突变体表型Mutations in barley and wheat EGT1 genes confer a striking root phenotype, where every root class adopts a steeper growth angle. 


结果2-EGT1编码蛋白及其自然变异EGT1 encodes an F-box and Tubby domain-containing protein that is highly conserved across plant species. Haplotype analysis found that natural allelic variation at the barley EGT1 locus impacts root angle. 



结果3-EGT1作用机制Gravitropic assays indicated that Hvegt1 roots bend more rapidly than wild-type. Transcript profiling revealed Hvegt1 roots deregulate reactive oxygen species (ROShomeostasis and cell wall-loosening enzymes and cofactors. ROS imaging shows that Hvegt1 root basal meristem and elongation zone tissues have reduced levels. Atomic force microscopy measurements detected elongating Hvegt1 root cortical cell walls are significantly less stiff than wild-type. 


结果4-EGT1表达模式In situ analysis identified HvEGT1 is expressed in elongating cortical and stele tissues, which are distinct from known root gravitropic perception and response tissues in the columella and epidermis, respectively.  


结论We propose that EGT1 controls root angle by regulating cell wall stiffness in elongating root cortical tissue, counteracting the gravitropic machinery’s known ability to bend the root via its outermost tissues. We conclude that root angle is controlled by EGT1 in cereal crops employing an antigravitropic mechanism. 


1.jpg


 摘 要 

在作物中,根的角度是一个作用于高效获取土壤中营养物质的关键性状。根的角度受到重力和反重力抵消(AGO)机制之间的竞争性作用。本文中,作者报道了一个根角度调控基因EGT1,该基因编码一个假定的AGO组分,其功能缺失突变能够增强根的向重力性。在大麦和小麦中突变EGT1基因能够引起显著的根表型改变,具体表现在几乎每个根都具有更陡的生长角度。EGT1基因编码一个含有F-box和Tubby结构域的蛋白,在不同植物物种间高度保守。单倍型分析显示,大麦EGT1位点上的自然等位变异影响了根的角度。重力实验显示Hvegt1突变体的根要比野生型弯曲得更快。转录图谱显示,Hvegt1突变体的根解除了对ROS内稳态、细胞壁松弛酶及辅因子的调控。ROS呈像显示,Hvegt1突变体根基部分生组织和伸长区组织的ROS积累水平降低。原子力显微镜测量显示,Hvegt1突变体中正在伸长的根皮层细胞明显比野生型更加坚硬,不容易弯曲。原位分析显示,HvEGT1在根中正在伸长的皮层细胞和中柱组织中表达,但是目前已知的根重力感知和响应因子分别在小柱和表皮组织中。作者提出,EGT1通过调控根中正在伸长的皮层细胞的细胞壁坚硬程度,抵消了目前已知的根向重力性机制通过根最外层组织来弯曲根的能力,从而控制根的生长角度。作者总结道,在谷类作物中EGT1基因通过一个反重力性机制,控制了根的角度。




 Malcolm Bennett 


个人简介:

1978-1983年,利物浦大学,学士;

1983-1986年,利物浦大学,博士。


研究方向:植物的根生长与发育。


doi: https://doi.org/10.1073/pnas.2201350119


Journal: PNAS

Published date: July 26, 2022


Cite:
Riccardo Fusi, Serena Rosignoli, Haoyu Lou, Giuseppe Sangiorgi, Riccardo Bovina, Jacob K. Pattem, Aditi N. Borkar, Marco Lombardi, Cristian Forestan, Sara G. Milner, Jayne L. Davis, Aneesh Lale, Gwendolyn K. Kirschner, Ranjan Swarup, Alberto Tassinari, Bipin K. Pandey, Larry M. York, Brian S. Atkinson, Craig J. Sturrock, Sacha J. Mooney, Frank Hochholdinger, Matthew R. Tucker, Axel Himmelbach, Nils Stein, Martin Mascher, Kerstin A. Nagel, Laura De Gara, James Simmonds, Cristobal Uauy, Roberto Tuberosa, Jonathan P. Lynch, Gleb E. Yakubov, Malcolm J. Bennett, Rahul Bhosale, Silvio Salvi. Root angle is controlled by EGT1 in cereal crops employing an antigravitropic mechanism. PNAS, 2022, 119(31): e2201350119. DOI: https://doi.org/10.1073/pnas.2201350119

p.s. 通讯往期精彩链接:

PNAS:紧实土壤中,乙烯通过ABA和生长素来抑制水稻根系伸长

Science:紧实土壤通过限制乙烯扩散来抑制植物根系生长

Nature Plants:植物根系周边水文环境影响侧根生长方向

Science:生长素响应因子ARF7作用于植物的根朝水方向的分支

Nature Communications:水稻OsAUX1基因低磷条件下促进根毛伸长




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