SKI-INTERACTING PROTEIN interacts with SHOOT MERISTEMLESS to regulate shoot apical meristem formation

第一作者：Ruiqi Li

第一单位：中国农业大学

第一通讯：Ligeng Ma

 Abstarct 

背景回顾：The shoot apical meristem (SAM), which is formed during embryogenesis, generates leaves, stems, and floral organs during the plant life cycle. SAM development is controlled by SHOOT MERISTEMLESS (STM), a conserved Class I KNOX transcription factor that interacts with another subclass homeodomain protein, BELL, to form a heterodimer, which regulates gene expression at the transcriptional level in Arabidopsis (Arabidopsis thaliana). Meanwhile, SKI-INTERACTING PROTEIN (SKIP), a conserved protein in eukaryotes, works as both a splicing factor and as a transcriptional regulator in plants to control gene expression at the transcriptional and post-transcriptional levels by interacting with distinct partners. 

茎尖分生组织（SAM）于胚胎发生过程中形成，在植物的生命周期中不断产生叶、茎和花器官。SAM的发育受到STM控制，而STM是一个保守的I类KNOX转录因子，与另一亚类的homeodomain蛋白BELL相互作用，形成异源二聚体，从转录层面上调节基因的表达水平。同时，SKIP是一个在真核生物中保守的蛋白，其在植物中既是剪接因子又是转录调节因子，通过与不同的互作蛋白相互作用，在转录和转录后层面上控制基因的表达水平。

结果1-stm与skip表型类似：Here, we show that, similar to plants with a loss of function of STM, a loss of function of SKIP or the specific knockout of SKIP in the SAM region resulted in failed SAM development and the inability of the mutants to complete their life cycle.

本文中，作者发现SKIP基因的功能缺失或者SAM区域SKIP的特定敲除会与STM基因功能缺失表现出类似的表型，即导致SAM发育缺陷，突变体无法完成其生命周期。

结果2-SKIP拯救stm表型：In comparison, Arabidopsis mutants that expressed SKIP specifically in the SAM region formed a normal SAM and were able to generate a shoot system, including leaves and floral organs. 

相比之下，在突变体的SAM区域特异表达SKIP基因能够使得突变体的SAM发育正常，并且能够正常产生包括叶和花器官在内的地上部分器官。

结果3-SKIP和STM互作：Further analysis confirmed that SKIP interacts with STM in planta and that SKIP and STM regulate the expression of a similar set of genes by binding to their promoters. 

进一步的分析证实，植物体内SKIP与STM能够互作，并且SKIP和STM通过结合目的基因的启动子，差不多控制同一组的基因的表达。

结果4-STM和ELF7互作：In addition, STM also interacts with EARLY FLOWERING 7 (ELF7), a component of Polymerase-Associated Factor 1 complex (Paf1c), and mutation in ELF7 exhibits similar SAM defects to that of STM and SKIP. 

此外，STM还会与Paf1c复合体组分之一ELF7蛋白发生互作，ELF7基因的功能缺失突变表现出与STM和SKIP突变相似的SAM缺陷。

结论：This work identifies a component of the STM transcriptional complex and reveals the mechanism underlying SKIP-mediated SAM formation in Arabidopsis.

本文的研究结果揭示了STM转录复合物的一个组成部分，并鉴定了由SKIP介导的拟南芥SAM形成的潜在分子调控机制。

** 马力耕  **

doi: https://doi.org/10.1093/plphys/kiac241

Journal: Plant Physiology

Published date: May 28, 2022

CITE

Ruiqi Li, Zhifeng Wei, Yan Li, Xudong Shang, Ying Cao, Liusheng Duan, Ligeng Ma. SKI-INTERACTING PROTEIN interacts with SHOOT MERISTEMLESS to regulate shoot apical meristem formation. Plant Physiology, 2022. DOI: https://doi.org/10.1093/plphys/kiac241