Ubiquitin Ligase OsRINGzf1 Regulates Drought Resistance by Controlling the Turnover of OsPIP2;1

第一作者：Shoujun Chen

第一单位：华中农业大学

通讯作者：Lijun Luo

 Abstarct 

背景回顾：Water is crucial for plant growth and survival. The trans-cellular water movement is facilitated by aquaporins that rapidly and reversibly modify water permeability. The abundance of aquaporins is regulated by its synthesis, redistribution, and degradation. However, the molecular mechanism of proteasomal degradation of aquaporins remains unclear. 

水对植物的生长和生存至关重要。水通道蛋白能够迅速且可逆地改变细胞的透水性，从而促进细胞间的水运动。水通道蛋白的丰度受其合成、再分配和降解的调节。然而，水通道蛋白的蛋白酶体降解分子调控机制尚不清楚。

主要研究：Here we demonstrate that a novel E3 ligase, OsRINGzf1, mediated the degradation of aquaporins in rice. 

本文中，作者鉴定到了一个新的E3连接酶OsRINGzf1，该酶介导了水稻中水通道蛋白的降解。

结果1-OsRINGzf1基因：OsRINGzf1 is the candidate gene from a drought related quantitative trait locus (QTL) on the long arm of chromosome 4 in rice (Oryza sativa) and encodes a Really Interesting New Gene (RING) zinc finger protein 1 gene. 

OsRINGzf1是水稻4号染色体长臂上干旱相关数量性状位点区域的一个候选基因，编码一个RING锌指蛋白。

结果2-OsRINGzf1下游：OsRINGzf1 possesses the E3 ligase activity, ubiquitinates and mediates OsPIP2;1 degradation, hence reducing its protein abundance. The content of OsPIP2;1 protein was decreased in OsRINGzf1 overexpression (OE) plants. The degradation of OsPIP2;1 was inhibited when treated with MG132. 

OsRINGzf1蛋白具有E3连接酶活性，能够泛素化OsPIP2;1并介导其降解，从而降低后者的蛋白丰度。OsPIP2;1蛋白的含量在OsRINGzf1过表达植物中降低。当用MG132进行处理时，能够抑制OsPIP2;1蛋白的降解。

结果3-OsRINGzf1基因功能：The OsRINGzf1 OE plants, with higher leaf related water content (LRWC) and lower leaf water loss rate (LWLR), exhibited enhanced drought resistance. Whereas, the RNAi and knockout plants of OsRINGzf1 were more sensitive to drought. 

OsRINGzf1过表达植株具有较高的叶片相关含水量和较低的叶片失水率，表现出较强的抗旱性。相反，OsRINGzf1基因的RNAi和敲除株系对干旱则更加敏感。

结论：Together, our data demonstrated that OsRINGzf1 positively regulates drought resistance through promoting the degradation of OsPIP2;1 to enhance water retention capacity in rice.

综上，本文的研究结果表明OsRINGzf1通过促进OsPIP2;1蛋白的降解来提高水稻的保水能力，从而正向调控水稻的抗旱性。

 罗利军 

doi: https://doi.org/10.1111/pbi.13857

Journal: Plant Biotechnology Journal

First Published: May 19, 2022

CITE

Shoujun Chen, Kai Xu, Deyan Kong, Lunying Wu, Qian Chen, Xiaosong Ma, Siqi Ma, Tianfei Li, Qi Xie, Hongyan Liu, Lijun Luo. Ubiquitin Ligase OsRINGzf1 Regulates Drought Resistance by Controlling the Turnover of OsPIP2;1. Plant Biotechnology Journal, 2022. DOI: https://doi.org/10.1111/pbi.13857