Gene regulatory networks shape developmental plasticity of root cell types under water extremes in rice

第一作者：Mauricio A. Reynoso

第一单位：加州大学河滨分校

通讯作者：Julia Bailey-Serres

 Abstarct 

背景回顾：Understanding how roots modulate development under varied irrigation or rainfall is crucial for development of climate-resilient crops. 

理解植物的根在不同的灌溉或降雨条件下如何调整发育对于开发具有较强的气候适应性的作物新品种至关重要。

主要研究：We established a toolbox of tagged rice lines to profile translating mRNAsand chromatin accessibility within specific cell populations. We used these to study roots in a range of environments: plates in the lab, controlled greenhouse stress and recovery conditions, and outdoors in a paddy. 

本文中，作者建立了一个标记水稻株系的工具包，用以对特定细胞类群内的正在翻译的mRNAs以及染色质可及性进行定量。作者利用该技术研究了根在不同环境条件下的响应，分别为实验室的培养皿中、温室条件下的胁迫处理与恢复、以及室外大田条件。

结果：Integration of chromatin and mRNA data resolves regulatory networks of the following: cycle genes in proliferating cells that attenuate DNA synthesis under submergence; genes involved in auxin signaling, the circadian clock, and small RNA regulation in ground tissue; and suberin biosynthesis, iron transporters, and nitrogen assimilation in endodermal/exodermal cells modulated with water availability. By applying a systems approach, we identify known and candidate driver transcription factors of water-deficit responses and xylem development plasticity. 

整合染色质和mRNA数据，作者揭示了以下几个方面的调控网络：植物在水淹胁迫下增殖细胞中影响DNA合成的周期基因；基本组织中参与生物素信号转导、生物钟以及小RNA调控的基因；内胚层/外胚层细胞中随水分可利用性的变化而被调节的木栓质生物合成、铁转运蛋白和氮同化。通过利用一个系统方法，作者鉴定了植物对缺水响应和木质素发育可塑性的已知和候选转录因子。

结论：Collectively, this resource will facilitate genetic improvements in root systems for optimal climate resilience.

综上，本文的研究结果为作物根系的遗传改良提供了宝贵的遗传资源，将有助于未来开发出最佳的气候适应性作物品种。

 Julia Bailey-Serres 

doi: https://doi.org/10.1016/j.devcel.2022.04.013

Journal: Developmental Cell

Published date: May 02, 2022