The single-cell stereo-seq reveals region-specific cell subtypes and transcriptome profiling in Arabidopsis leaves

第一作者：Keke Xia

第一单位：华大基因

通讯作者：Xun Xu

 Abstarct 

背景回顾：Understanding the complex functions of plant leaves requires a thorough characterization of discrete cell features. Although single-cell gene expression profiling technologies have been developed, their application in characterizing cell subtypes has not been achieved yet. 

理解植物叶片的复杂功能需要对不同细胞类型的完全刻画。尽管目前已经开发出了单细胞基因表达谱技术，但是该技术还不能用来区分细胞的亚型。

主要研究：Here, we present scStereo-seq (single-cell spatial enhanced resolution omics sequencing) that enabled us to show the bona fide single-cell spatial transcriptome profiles of Arabidopsis leaves. 

本文中，作者报道了一种具有高空间分辨率的单细胞测序技术scStereo-seq，并通过对拟南芥的叶片进行了单细胞空间转录组测序。

结果：Subtle but significant transcriptomic differences between upper and lower epidermal cells have been successfully distinguished. Furthermore, we discovered cell-type-specific gene expression gradients from the main vein to the leaf edge, which led to the finding of distinct spatial developmental trajectories of vascular cells and guard cells. 

利用scStereo-seq，作者能够区分拟南芥叶片上、下表皮之间细微但十分显著的转录组差异。此外，作者还发现了从主脉到叶边缘的细胞类型特异性基因表达梯度，从而揭示了维管细胞和保卫细胞的不同空间发育轨迹。

结论：Our study showcases the importance of physical locations of individual cells for exerting complex biological functions in plants and demonstrates that scStereo-seq is a powerful tool to integrate single-cell location and transcriptome information for plant biology study.

本文的研究发现植物中单个细胞的物理空间位置对于其发挥复杂生物学功能具有重要作用，同时还揭示了scStereo-seq技术是植物生物学研究中整合单细胞位置信息和转录组信息的有力工具。

 徐 迅 

doi: https://doi.org/10.1016/j.devcel.2022.04.011

Journal: Developmental Cell

Published date: May 04, 2022